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本研究从梭梭中克隆了2条NAC基因,分别命名为序列Ha NAC3(KU534095)和Ha NAC4(KU534-096)。序列分析显示Ha NAC3基因编码一条242个氨基酸的多肽,Ha NAC4基因编码一条287个氨基酸的多肽,并且编码的蛋白都为亲水性蛋白。经过同源性比对分析发现2条序列都含有典型的NAC结构域,属于NAC转录因子家族。组织特异性表达分析结果显示Ha NAC3序列在梭梭根部有较高强度的表达;Ha NAC4在梭梭种子中表达量较高。干旱胁迫下,Ha NAC3和Ha NAC4呈现出不同的表达方式,本研究有助于为进一步分析梭梭NAC家族基因功能提供帮助。
In this study, two NAC genes were cloned from Haloxylon ammodendron and named as sequences Ha NAC3 (KU534095) and Ha NAC4 (KU534-096) respectively. Sequence analysis showed that the Ha NAC3 gene encodes a 242 amino acid polypeptide, and the Ha NAC4 gene encodes a 287 amino acid polypeptide, and the encoded protein is both a hydrophilic protein. After homology analysis, it was found that both sequences contained a typical NAC domain and belonged to the NAC transcription factor family. Tissue-specific expression analysis showed that Ha NAC3 was highly expressed in the roots of Haloxylon ammodendron and Ha NAC4 was highly expressed in Haloxylon ammodendron seeds. Under drought stress, Ha NAC3 and Ha NAC4 displayed different expression patterns, and this study may be helpful to further analyze the function of NAC family of Haloxylon ammodendron.