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冷藏和贮存生殖细胞,以实行人工授精、体外授精、胚胎移植和人胚的体外培养。精液的冷藏,国外已有使用。一般将取得的精液,加入葡萄糖、甘油、卵黄等以保护精子,并将酸碱度调节在7．2~7．4之间,再置入-196℃(液氮温度)的低温环境便可。冷藏十年以上复温仍可使女性怀孕。由于畸形或发育异常的精子,易在冷藏过程中被淘汰掉,所以发现用冷藏精液使卵子受孕而出生的孩子,发生流产和胎儿畸形等情况的反而更少。
Refrigeration and storage of germ cells, in order to implement artificial insemination, in vitro fertilization, embryo transfer and in vitro culture of human embryos. Semen refrigeration, has been used abroad. General semen will be added to glucose, glycerol, egg yolk and so on to protect the sperm, and the pH adjusted between 7.2 to 7.4, and then placed at -196 ℃ (liquid nitrogen temperature) of the low temperature environment can be. Refrigeration for more than a decade can still make women pregnant. Due to deformity or abnormal development of sperm, easy to be eliminated in the process of cold storage, it was found that the use of refrigerated sperm to conceive eggs born children, abortion and fetal malformations but less.