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目的:研究芪天复方中主要活性成分芍药苷在大鼠肠吸收动力学的特征,为芪天复方口服制剂设计提供依据。方法:采用单向灌流模型,HPLC法测定灌流液中芍药苷的含量,考察药物浓度、灌流液pH以及吸收部位对芍药苷吸收的影响。结果:芪天复方中芍药苷在30.40～120.80mg·L-1范围内小肠吸收速率常数Ka及表观吸收系数Papp的差异无显著性(P>0.05);在十二指肠、空肠、回肠及结肠的吸收速率常数Ka及表观吸收系数Papp差异无显著性(P>0.05)。结论:芪天复方中主要活性成分芍药苷在大鼠小肠中的吸收呈一级动力学过程,为被动扩散机制;芍药苷在大鼠各肠段均有较好的吸收。
OBJECTIVE: To study the absorption kinetics of paeoniflorin in the main active ingredient of Qitian Compound, and to provide basis for the design of compound prescription of Qitian. Methods: The content of paeoniflorin in perfusate was determined by one-way perfusion model. The concentration of paeoniflorin in the perfusate was determined by HPLC. The effects of drug concentration, pH of the perfusate and absorption site on the absorption of paeoniflorin were investigated. Results: There was no significant difference of the intestinal absorption rate constant Ka and the apparent absorption coefficient Papp in the range of 30.40-120.80 mg · L-1 for the paeoniflorin of Qitian Compound (P> 0.05). In the duodenum, jejunum and ileum And colon absorption rate constant Ka and apparent absorption coefficient Papp no significant difference (P> 0.05). CONCLUSION: Paeoniflorin, the major active component in Qitian Compound, is in a first-order kinetic process and is a passive diffusion mechanism. Paeoniflorin has a good absorption in each intestine of rats.