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1992年6月,采用WHO接触简法,按照1991年全国蚊类抗性监测网提供的剂量方法,检测了中华按蚊成虫对溴氰菊酯和DDT抗性,按机率值回归分析法处理检测结果。以0.01％的溴氰菊酯接触25分钟为区分剂量,其死亡率为80.72％;经0.01％的浓度作64、32、16、8分钟系列时间,结果LT_(50)为22分钟,95％可信限为19——24分钟(X~2=1.373,P>0.05)。以4％的DDT接触1小时为区分剂量,其死亡率为78.38％;按4％、2％、1％、0.5％均接触1小时作系列浓度,结果LC50为3.1％,95％可主限为2.58——3.72％,(X~2=3.242,P>0.05)。检测结果表明我市中华按蚊对溴氰菊酯、DDT具有初级抗性和肯定抗性。
In June 1992, the WHO method of contact briefing was used to detect deltamethrin and DDT resistance of adult mosquitoes of Anopheles sinensis in accordance with the dose method provided by National Anti-mosquito Network in 1991, and the detection rate was tested by regression analysis result. 0.01% deltamethrin exposure for 25 minutes for the division dose, the mortality rate was 80.72%; 0.01% concentration for 64,32,16,8 minutes series time, the result LT_ (50) is 22 minutes, 95% The confidence limit was 19- 24 minutes (X ~ 2 = 1.373, P> 0.05). The 4%, 2%, 1%, 0.5% contact time of 1 hour was used as the differentiating dose with the 4% DDT exposure for one hour. The mortality was 78.38%. The results showed that LC50 was 3.1% and 95% Was 2.58 - 3.72%, (X ~ 2 = 3.242, P> 0.05). The test results showed that Anopheles sinensis had primary resistance and positive resistance to deltamethrin and DDT.