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目的分析新疆地区人类免疫缺陷病毒1型(human immunodeficiency virus type 1,HIV-1)/丙型肝炎病毒(hepatitis C virus,HCV)混合感染者体内HCV各基因型在6个月时间间隔前后基因变化规律,为针对HCV制定预防治疗策略和后续的研究提供基础。方法对新疆地区2013年4月确认为HIV-1抗体阳性的102例感染者进行HCV抗体检测,HCV抗体阳性的研究对象在6个月后(2013年10月)进行第二次采血。对HCV抗体阳性的2次标本抽提血浆病毒RNA,反转录后扩增HCV 5′UTR基因,将所得到的序列与HCV的国际标准株进行比较,确定被检样本的HCV基因型。基于2次采样毒株的序列变异,使用MEGA 6软件计算其基因离散率,使用Beast v1.8.0软件分析起源时间和进化速率。结果 102例HIV-1感染者中有89例(87.25%)HCV抗体阳性,其中成功扩增出HCV 5′UTR基因2次序列的有77例(77/89,86.52%)。HCV 5′UTR基因片段分型结果显示有4种基因型:1a(9/77,11.69%),1b(31/77,40.26%),3a(24/77,31.17%),3b(13/77,16.88%)。HCV的1b基因型6个月后样本的组内基因离散率(0.025±0.007)%明显大于首次采样的(0.001±0.001)%,其他3种基因型的基因离散率在6个月后无明显变化。HCV的5′UTR基因平均进化速率为1.62×10-3(95%HPD 1.52×10-3~5.38×10-3)替换/(位点·年),各基因亚型之间的进化速率相差1~3倍,3a亚型祖先株形成时间最为久远。结论新疆地区HIV-1感染者中静脉吸毒人群合并HCV感染率高,HCV的3种基因型(1a、3b、3a)在HIV-1感染者体内稳定存在,总体变异不大,但1b基因型随着时间的推移相对比较活跃。
Objective To analyze the genetic variation of HCV genotypes in human immunodeficiency virus type 1 (HIV-1) / hepatitis C virus (HCV) co-infected patients in Xinjiang before and after 6 months interval Rules, in order to develop prevention and treatment of HCV strategy and follow-up study provide the basis. Methods HCV antibodies were detected in 102 HIV-1 positive individuals in Xinjiang in April 2013. HCV antibody-positive subjects received a second blood collection 6 months later (October 2013). Plasma HCV RNA was extracted from two specimens positive for HCV antibody and reverse transcribed to amplify the HCV 5’UTR gene. The obtained sequence was compared with the international standard strain of HCV to determine the HCV genotype of the test sample. Based on the sequence variation of the 2 sampled strains, the genetic divergence was calculated using the MEGA 6 software and the origination time and evolution rate were analyzed using the Beast v1.8.0 software. Results Of the 102 HIV-1 infected patients, 89 (87.25%) were positive for HCV antibody. 77 (89.86.52%) of them successfully amplified two sequences of HCV 5’UTR gene. The genotyping results of HCV 5’UTR gene revealed four genotypes: 1a (9/77, 11.69%), 1b (31/77, 40.26%), 3a (24/77, 31.17% 77, 16.88%). The genotype distribution of genotype 1b in HCV genotype 1b after 6 months was significantly (0.025 ± 0.007)% greater than that of the first sample (0.001 ± 0.001)%, while the genetic divergence of the other three genotypes was not obvious after 6 months Variety. The average rate of evolution of HCV 5’UTR gene was 1.62 × 10-3 (95% HPD 1.52 × 10-3 ~ 5.38 × 10-3), and the difference in the evolution rates of each gene subtype 1 to 3 times, 3a subtype ancestral strains of the oldest. Conclusions The prevalence of HCV infection among HIV-1 infected persons in Xinjiang is high. HCV genotypes (1a, 3b, 3a) are stable in HIV-1 infected individuals with little overall variation, but 1b genotype As time goes by relatively active.