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目的:研究和阐明中枢组胺对东莨菪碱所致大鼠(空间)记忆障碍的作用机制.方法:采用迷宫学习的程序研究大鼠的空间记忆,并利用高效液相法测定脑内组胺含量.结果:侧脑室内注射组胺(100,200 ng)、2-β-噻唑乙胺(200 ng)及4-[4’-(环己氨基硫代甲酰基哌啶)]-4H-咪唑(50μg)或腹腔内注射组胺酸(1000 mg/kg)均可对抗东莨菪碱所致的记忆障碍.相反,4-甲基组胺(50-200 ng)却无明显作用.组胺(200 ng)和组胺酸(1000 mg/kg)均可有效地增加大脑皮层、海马及下丘脑中的组胺含量.结论:中枢组胺可以明显改善东莨菪碱引起的大鼠空间记忆障碍,其作用主要与H_1、H_3受体相关.
OBJECTIVE: To study and clarify the mechanism of central histamine on scopolamine-induced memory impairment in rats.Methods: The spatial memory of rats was studied by maze learning program, and the content of histamine in brain was determined by HPLC. RESULTS: Intracerebroventricular injection of histamine (100,200 ng), 2-β-thiazolyl amine (200 ng), and 4- [4 ’- (cyclohexylthiocarbamylpiperidine) Or intraperitoneal injection of histidine (1000 mg / kg) could counteract the memory impairment caused by scopolamine, whereas 4-methylhistamine (50-200 ng) showed no significant effect.Histamine (200 ng) and group (1000 mg / kg) can effectively increase the histamine content in cerebral cortex, hippocampus and hypothalamus.Conclusion: Central histamine can significantly improve scopolamine-induced spatial memory impairment in rats, and its role is mainly related to H 1, H 3 Receptor related.