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目的探讨苦参素对胃癌细胞株SGC-7901的抑制增殖效应及凋亡诱导作用。方法用苦参素处理SGC-7901细胞,采用MTT法检测苦参素对细胞的抑制作用,透射电镜观察微丝的变化,流式细胞术检测细胞凋亡率和细胞周期。结果在苦参素作用下,SGC-7901细胞呈凋亡改变。细胞凋亡的同时,细胞的活力下降,微丝几近消失,不同浓度的苦参素均可导致细胞凋亡和细胞周期阻抑。结论苦参素能抑制胃癌细胞增殖,能诱导胃癌细胞凋亡。
Objective To investigate the inhibitory effect of oxymatrine on gastric cancer cell line SGC-7901 and the induction of apoptosis. Methods Oxymatrine was used to treat SGC-7901 cells. The inhibitory effect of oxymatrine on the cells was detected by MTT assay. The changes of microfilaments were observed by transmission electron microscopy. The apoptosis rate and cell cycle were detected by flow cytometry. Results Under the action of oxymatrine, SGC-7901 cells showed apoptosis changes. At the same time of apoptosis, the viability of cells decreased and the fibroids almost disappeared. Different concentrations of oxymatrine could lead to apoptosis and cell cycle arrest. Conclusion Oxymatrine can inhibit gastric cancer cell proliferation and induce gastric cancer cell apoptosis.