论文部分内容阅读
目的:探讨蒙药槟榔十三味丸对慢性应激抑郁模型大鼠神经-内分泌-免疫功能的影响及治疗抑郁症的机制。方法:将Wistar雄性大鼠,根据蔗糖水消耗量及体重随机分为正常对照组、模型组、氟西汀组(3.3 mg·kg-1)、槟榔十三味丸低、中、高剂量组(0.25,0.5,1 g·kg-1),每组10只。除正常对照组外,其余大鼠均采用慢性轻度不可预见性应激结合孤养方法制备抑郁模型,造模同时灌胃给药,每日1次,连续给药28 d。实验结束后,迅速断头取血,分离血清,采用酶联免疫吸附法(ELISA)检测促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)、皮质酮(CORT)、三碘甲状腺原氨酸(T3)、四碘甲状腺原氨酸(T4)、促甲状腺激素(TSH)、白介素-2(IL-2)、白介素-6(IL-6)的含量,并取胸腺、脾脏,计算胸腺指数、脾脏指数。结果:与正常对照组比较,模型组大鼠血清CRH,ACTH,CORT,T3,IL-6含量明显升高(P<0.01),TSH含量、脾脏指数明显降低(P<0.01);与模型组比较,氟西汀组和槟榔十三味丸大鼠血清CRH,ACTH,CORT,T3,IL-6含量明显降低(P<0.01或P<0.05)、TSH含量明显升高(P<0.05或P<0.01),槟榔十三味丸组大鼠脾脏指数明显升高(P<0.01),尤其槟榔十三味丸高剂量组作用显著。结论:槟榔十三味丸能调节抑郁模型大鼠下丘脑-垂体-肾上腺轴和下丘脑-垂体-甲状腺轴功能,以及免疫功能,这可能是该复方抗抑郁作用的机制之一。
Objective: To investigate the effects of Mongolian medicine arecajisanweiwei pill on the neuroendocrine-immune function of chronic stress depression model rats and the mechanism of depression treatment. Methods: Wistar male rats were randomly divided into normal control group, model group, fluoxetine group (3.3 mg · kg-1), low-medium-high-dose betel nutsticks (0.25,0.5,1 g · kg-1), 10 in each group. Except for the normal control group, the rest of the rats were treated with mild mild unpredictable stress combined with solitary method to make depression model. The model was given intragastrically once a day for 28 days. After the experiment, blood was rapidly decapitated, and serum was separated. Corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH), corticosterone (CORT), triiodothyroid (T3), T4, TSH, IL-2 and IL-6 in the thymus, spleen, thymus, Calculate thymus index, spleen index. Results: Compared with the normal control group, the levels of serum CRH, ACTH, CORT, T3 and IL-6 in the model group were significantly increased (P <0.01) and the content of TSH and spleen index in the model group were significantly decreased The levels of serum CRH, ACTH, CORT, T3 and IL-6 in the fluoxetine and betel nut decoction groups were significantly decreased (P <0.01 or P <0.05) and TSH levels were significantly increased (P <0.05 or P < 0.01). The spleen index of betel nut group was significantly higher than that of betel nut group (P <0.01). Conclusion: Arecajisan can regulate the function of hypothalamus-pituitary-adrenal axis and hypothalamus-pituitary-thyroid axis and the immune function in depression model rats, which may be one of the mechanisms of the antidepressant effect of betel nut.