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对五个籼稻Ｆ１代杂种及其中的三个亲本品种进行花药培养，考察了多个因子对籼稻花药培养效率的影响。结果表明，在所有的供试材料中，ＫＤＭＬ１０５／ＳＰＲ９０在相同条件下对花药培养最敏感，其最高的愈伤组织的诱导率可达２４．４５％，Ｎ６和Ｍ８培养基均可用于籼稻花药愈伤组织诱导，但Ｍ８的愈伤组织诱导率高于Ｎ６。固体的诱导培养基优于液体培养基。ＡｇＮＯ３作为一种抗乙烯组分，在Ｎ６和Ｍ８培养基中的作用效果不明显。缩短穗子的表面消毒时间能显著降低消毒对花药的伤害。推荐的表面消毒程序为喷７０％的酒精０．５—１分钟，迅速用无菌水冲洗两次即可。本试验的绿苗再生率仍较低，８个供试品程（组合）中，只有ＫＤＭＬ１０５／ＳＰＲ９０和ＳＰＲ９０分别得到６和２８株绿苗，最高的绿苗频率为０～７３％（以花药为基础）。
Five indica rice F1 hybrids and three of them were cultured in anther culture. The effects of several factors on the anther culture efficiency of indica rice were investigated. The results showed that KDML105 / SPR90 was most sensitive to anther culture under the same conditions, and the highest callus induction rate was 24.45%. N6 and M8 mediums could be used for indica anthers Callus induction, but M8 callus induction rate higher than N6. Solid induction medium is better than liquid medium. The effect of AgNO3 as an anti-ethylene component in N6 and M8 medium is not obvious. Shortening the surface of the spike disinfection time can significantly reduce disinfection of anther damage. The recommended surface disinfection procedure is to spray 70% alcohol for 0.5-1 minutes and rinse twice with sterile water quickly. The regeneration rate of green plantlets in this experiment was still low. Only 6 and 28 green plantlets were obtained from KDML105 / SPR90 and SPR90, respectively, and the highest frequency of green plantlets was 0-73% (using anther As the basis).