论文部分内容阅读
研究了13种提取的和3种人工合成的天然产物对白血病或腹水型肝癌细胞DNA合成的抑制作用及抑制机理,并研究了它们对新生儿脐带、血淋巴细胞姊妹染色单体交换(SCE)的影响。抗癌药往往具有致突变或癌变的作用,SCE是测定致突或致癌作用的最灵敏手段,测定诱发SCE的最低浓度有助于控制用药量的上限,可供临床用量参考。所用天然物均由兰州大学有机化学研究所从植物中提取或合成,并进行结构鉴定。一种试剂对癌细胞DNA合成的半抑制浓度(IC_(50)~-)越小,意味着抗癌能力越强。IC_(50)~-介于1～25mg/L的有天然的和合成的异三尖杉酯碱、去氢骆驼蓬碱、细叶骆驼蓬总碱(脂溶及水溶)和多被银莲花素A等6种,其中以天然的异三尖杉酯碱作用最强,lC_(50)~-仅1mg/L。而脱氧鸭嘴花碱和高车前素的IC_(50)~-很大,分别为400和116mg/L,IC_(50)~-介乎33～66mg/L的有槲皮
The inhibitory and inhibitory mechanisms of 13 extracted and 3 synthetic natural products on DNA synthesis of leukemia or ascitic hepatocellular carcinoma cells were studied, and their sister chromatid exchange (SCE) on umbilical cord and blood lymphocytes was studied. Impact. Anticancer drugs often have mutagenic or cancerous effects. SCE is the most sensitive method for determining the oncogenic or carcinogenic effect. The determination of the lowest concentration of induced SCE is helpful to control the upper limit of the drug dosage and can be used as a reference for clinical use. The natural products used were all extracted or synthesized from plants by the Institute of Organic Chemistry, Lanzhou University, and their structures were identified. The smaller the semi-inhibitory concentration (IC_(50)~-) of an agent on the DNA synthesis of cancer cells, the stronger the anti-cancer ability. IC_(50)~- between 1 and 25 mg/L of natural and synthetic isoharringtonine, hydrogenated harmine, total alkaloids (liposoluble and water-soluble), and mostly anemones There are six kinds of A, among which the natural heteroharringtonine has the strongest effect, lC_(50)~-only 1mg/L. The IC_(50)~- is very large for deoxygenated duck bilobaine and high carcropin, 400 and 116 mg/L, and IC_(50)~- between 33 and 66 mg/L.