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目的通过模拟汽车尾气持续暴露伤害,探讨汽车尾气对雄性小鼠生精组织的损伤和功能的影响。方法将20只雄性C57BL/6小鼠按随机数字表法分成暴露组和对照组,每组10只。在相同实验环境中对暴露组实施稳定的尾气暴露后,采用气体成分检测、血气分析方法从缺氧角度比较2组差异;通过脏器系数、HE染色和计算机辅助精子分析等方法,从形态和功能的角度比较对照组与暴露组的差异;检测血浆及生精组织一氧化氮(NO)和总抗氧化能力(total antioxidant capacity,T-AOC),从氧化水平比较对照组与暴露组的差异。结果装置内成分检测发现,从暴露开始到暴露结束氧含量、二氧化硫含量、PM2.5含量恒定,暴露组小鼠进行血气分析发现无缺氧表现,与对照组比较差异无统计学意义(P>0.05)。与对照组比较,暴露组睾丸、附睾脏器系数差异无统计学意义(P>0.05),睾丸组织生精细胞排列稀疏且生精层明显减少,同时出现空泡样改变,管腔内精子减少;计算机辅助精子分析发现暴露组精子数量和质量明显下降,差异有统计学意义(P<0.05)。与对照组比较,暴露组的血浆及生精组织T-AOC水平明显下降,血浆及生精组织NO水平明显升高,差异有统计学意义(P<0.05)。结论汽车尾气的持续暴露损伤了雄性C57BL/6小鼠的生精组织和功能,而这一损伤可能与血浆和生精组织氧化水平的改变相关。
Objective To investigate the effects of vehicle exhaust on the damage and function of spermatogenic tissues in male mice by simulating the sustained exposure of vehicle exhaust. Methods Twenty male C57BL / 6 mice were divided into exposure group and control group according to random number table method, with 10 in each group. After exposing exposed groups to the same experimental environment, gas composition and blood gas analysis were used to compare two groups of differences from the point of view of lack of oxygen. By means of organ coefficient, HE staining and computer-aided sperm analysis, The differences of the control group and the exposed group were compared between the control group and the exposed group. The levels of nitric oxide (NO) and total antioxidant capacity (T-AOC) in plasma and spermatogenesis were measured. . Results In the device, the oxygen content, the content of sulfur dioxide and the content of PM2.5 were constant from the beginning of exposure to the end of exposure. The blood gas analysis of exposed mice showed no hypoxia, and there was no significant difference compared with the control group (P> 0.05). Compared with the control group, the organ coefficient of testis and epididymis in the exposed group had no significant difference (P> 0.05), the spermatogenic cells in the testis were sparsely arranged and the spermatogenic layer was significantly reduced, while the vacuole-like changes and the decrease of intraluminal sperm ; Computer-assisted sperm analysis found that the number and quality of sperm in the exposed group decreased significantly, the difference was statistically significant (P <0.05). Compared with the control group, the levels of T-AOC in the plasma and spermatogenic tissue of the exposed group were significantly decreased, while the levels of NO in the plasma and spermatogenic tissue were significantly increased (P <0.05). Conclusion The continuous exposure of vehicle exhaust damaged the spermatogenic tissues and function of male C57BL / 6 mice, which may be related to the changes of plasma and spermatogenesis.