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目的研究二甲基氯化锡(DMT)对大鼠肾脏氢钾-ATP酶(HKA酶)和钠钾-ATP酶(NKA酶)活力的影响。方法 (1)离体实验:取5只无特定病原体(SPF)级健康雌性SD大鼠肾组织,以0.90%氯化钠溶液制成匀浆液,加入质量浓度为1.0 g/L的DMT配制成终浓度分别为0、1、25、125和625 mg/L的肾匀浆液,采用酶标仪检测HKA酶和NKA酶活力。(2)体内实验:将40只SPF级健康SD大鼠随机分为对照组和染毒组,每组20只,雌雄各半;染毒组大鼠予16.000 mg/kg体质量DMT一次性腹腔注射染毒,对照组大鼠予等体积的0.90%氯化钠溶液,分别于染毒后1和24 h处死;摘取肾脏制备肾匀浆液,采用酶联免疫吸附实验检测肾脏组织中HKA酶和NKA酶活力;采集腹主动脉血,检测血清中钾离子(K~+)、钠离子(Na~+)和氯离子(Cl~-)水平。结果 (1)离体实验:DMT可抑制大鼠肾脏组织中HKA酶活力,随着DMT染毒剂量增加,其对HKA酶活力的抑制作用越明显,呈剂量-效应关系(P<0.01);但DMT对大鼠肾脏组织中NKA酶活力无影响(P>0.05)。(2)体内实验:染毒组大鼠24 h时间点体质量低于该时间点对照组(P<0.01);染毒组大鼠肾脏组织中HKA酶活力低于对照组(P<0.01);大鼠肾脏组织中NKA酶活力以及血清中K~+、Na~+和Cl-水平在染毒处理、染毒时间的主效应及其交互效应上均无统计学意义(P>0.05)。结论 DMT可抑制肾匀浆HKA酶活力,但对肾匀浆NKA酶活力影响不明显。
Objective To investigate the effect of dimethyl tin chloride (DMT) on the activity of potassium-ATPase (HKA) and sodium-potassium-ATPase (NKA) in rat kidney. Methods (1) In vitro experiments: Five healthy female Sprague-Dawley (SD) rats without specific pathogen (SPF) were taken from the kidney and homogenized with 0.90% sodium chloride solution. DMT supplemented with 1.0 g / L of DMT The final concentration of 0, 1, 25, 125 and 625 mg / L of renal homogenate, using the microplate reader HKA enzyme and NKA enzyme activity. (2) In vivo experiments: 40 SPF healthy SD rats were randomly divided into control group and exposure group, 20 rats in each group, male and female were divided into two groups. The rats in the exposure group were given a single intraperitoneal injection of 16.000 mg / kg DMT The rats in the control group were given an equal volume of 0.90% sodium chloride solution and sacrificed at 1 and 24 h after exposure respectively. The kidneys were harvested to prepare kidney homogenate, and the enzyme linked immunosorbent assay And NKA activity were measured. The abdominal aorta blood was collected and the levels of K +, Na + and Cl ~ - were detected. Results (1) In vitro, DMT could inhibit the activity of HKA in rat kidney tissue. With the increase of DMT dose, the inhibitory effect on HKA activity was more obvious with a dose - effect relationship (P <0.01). But DMT has no effect on NKA activity in rat kidney (P> 0.05). (2) In vivo, the body weight of rats in the exposed group was lower than that of the control group at 24 h (P <0.01). The activity of HKA in the kidney of the treated group was lower than that of the control group (P <0.01) The activity of NKA and the levels of K ~ +, Na ~ + and Cl- in serum of kidney tissue of rats were not significantly different between the exposure time and the exposure time (P> 0.05). Conclusion DMT can inhibit HKA enzyme activity of kidney homogenate, but it has no obvious effect on NKA enzyme activity of renal homogenate.