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目的:建立同时分析测定明目上清片中4个活性成分(柠檬烯、薄荷酮、薄荷脑和胡薄荷酮)的方法。方法:采用GC法,色谱柱为DB-1701毛细管色谱柱(30 m×0.3 mm,0.25μm),柱温采用程序升温(80℃保持1 min,5℃.min-1上升至120℃,保持1 min,再以50℃.min-1的速率升温至220℃,保持2 min);进样口温度:180℃;检测器:FID;检测器温度:250℃。结果:柠檬烯、薄荷酮、薄荷脑和胡薄荷酮线性范围分别为0.0103～0.507 mg·mL-1(r=0.9997)、0.0105～0.501 mg·mL-1(r=0.9999)、0.0082～0.412 mg·mL-1(r=0.9991)、0.0085～0.404 mg·mL-1(r=0.9998);平均加样回收率(n=6)分别为99.1%、97.6%、98.7%、98.3%,RSD分别为1.2%、1.8%、1.4%、1.5%。结论:该方法可用于明目上清片的质量检测。
Objective: To establish a method for the simultaneous determination of four active ingredients (limonene, menthone, menthol and pulegone) in the eyesight supernatant of Mingmu. Methods: GC-MS method was used. The column was DB-1701 capillary column (30 m × 0.3 mm, 0.25 μm). The temperature of the column was kept at 80 ℃ for 1 min and 5 ℃ .min- 1 min, and then heated to 220 ℃ at 50 ℃ .min-1 for 2 min); Inlet temperature: 180 ℃; Detector: FID; Detector temperature: 250 ℃. Results: The linear ranges of limonene, menthone, menthol and menthone were 0.0103-0.507 mg · mL -1 (r = 0.9997), 0.0105-0.501 mg · mL -1 (r = 0.9999), 0.0082-0.412 mg · (n = 6) were 99.1%, 97.6%, 98.7% and 98.3%, respectively, with RSD of 1.2%, 1.8%, 1.4%, 1.5%. Conclusion: The method can be used for the quality inspection of the eyesight supernatant.