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目的 :建立一个比较实用且成型时间短的地塞米松诱导大鼠胸腺细胞凋亡模型。方法 :给幼龄大鼠 ( 4周～ 5周 )腹腔注射地塞米松 ( 0 0 2 g/kg) ,采用形态学 (光镜和电镜 )、DNA琼脂糖凝胶电泳、流式细胞光度分析等方法研究 3、 6、 9、 1 5、 2 4h等不同注射时间胸腺细胞凋亡变化。结果 :在 1 5h内 ,地塞米松诱导的大鼠胸腺细胞凋亡百分率随时间延长 ,凋亡发生率从 6 2 %逐渐增至 58 5% ;注射地塞米松 1 5h ,在光镜和电镜下均观察到胸腺细胞凋亡的典型形态变化 ;DNA琼脂糖凝胶电泳呈现梯形条带 ;流式细胞光度分析可见凋亡峰出现。结论 :幼龄大鼠腹腔注射地塞米松 ( 0 0 2 g/kg) 1 5h可成功地诱导出一个比较理想的体内胸腺细胞凋亡模型。该模型成型时间短 ,适用于凋亡调控药物及其作用机理的研究。
Objective: To establish a more practical and short forming time of dexamethasone induced rat thymocyte apoptosis model. Methods: Dexamethasone (0 2 g / kg) was intraperitoneally injected into young rats (4 weeks to 5 weeks). Morphological (light and electron microscopy), DNA agarose gel electrophoresis and flow cytometry Methods such as 3, 6, 9, 1 5, 2 4h at different time of injection of thymus apoptosis changes. Results: The percentage of apoptotic thymocytes induced by dexamethasone in rats increased with time and the rate of apoptosis increased gradually from 62% to 58 5% within 15 h. After dexamethasone injection for 15 h, The morphological changes of thymocyte apoptosis were observed under the conditions of DNA ladder. DNA ladder showed a ladder-shaped band. The apoptotic peak appeared by flow cytometry. CONCLUSION: An ideal model of in vivo thymocyte apoptosis is successfully induced in young rats by intraperitoneal injection of dexamethasone (0 0 2 g / kg) for 15 h. The model has short forming time and is suitable for the study of apoptosis regulating drugs and its mechanism of action.