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目的探讨人脂肪间充质干细胞向血管内皮细胞诱导分化前后microRNA(miRNA)的差异表达,预测其靶基因。方法对人脂肪间充质干细胞诱导成的血管内皮细胞进行鉴定后,提取人脂肪间充质干细胞向血管内皮细胞诱导分化前后的miRNA,微阵列芯片检测表达谱的变化,对有显著差异的miRNA进行实时荧光定量PCR验证,通过TargetScan、Miranda、PITA、RNAhybrid和microTar五个数据库预测靶基因。结果筛选、验证出四个显著差异表达的miRNA。其中,miR-29b-3p和miR-5096为显著上调,miR-143-3p和miR-145-5p为显著下调。预测到OCT4、SOX2、KLF4、TGFB2、IGF1、TAF11、TMEM169、UHRF1和OSBPL6等相关靶基因。结论人脂肪间充质干细胞向血管内皮细胞诱导分化前后存在显著差异性表达的miRNA,提示这些miRNA在其分化中有重要的调控作用。
Objective To investigate the differential expression of microRNAs (miRNAs) before and after human adipose-derived mesenchymal stem cells differentiate into vascular endothelial cells and predict the target genes. Methods The human vascular endothelial cells induced by human adipose-derived mesenchymal stem cells were identified and the changes of miRNA and microarray microarray were detected before and after human adipose-derived mesenchymal stem cells were induced to differentiate into vascular endothelial cells. Real-time quantitative PCR was used to validate the target genes by five databases: TargetScan, Miranda, PITA, RNAhybrid and microTar. The results of screening, validation of four significantly differentially expressed miRNA. Among them, miR-29b-3p and miR-5096 were significantly upregulated, miR-143-3p and miR-145-5p were significantly downregulated. The related target genes of OCT4, SOX2, KLF4, TGFB2, IGF1, TAF11, TMEM169, UHRF1 and OSBPL6 are predicted. Conclusions There are significant differentially expressed miRNAs before and after human adipose-derived mesenchymal stem cells differentiate into vascular endothelial cells, suggesting that these miRNAs play an important regulatory role in their differentiation.