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目的:研究解毒通络注射液(Jiedu Tongluo injection,JDTL)对缺血性再灌注大鼠脑组织含水量变化以及细胞间黏附分子-1(ICAM-1)、血管细胞间黏附分子-1(VCAM-1)、E-选择素(E-selectin)、基质金属蛋白酶-9(MMP-9)表达的影响,探讨其对脑缺血再灌注损伤保护作用的机制。方法:线栓法建立大鼠大脑中动脉缺血再灌注模型,干-湿重法检测脑组织含水量;采用比色法测定脑组织中伊文思蓝(Evans blue,EB)含量反映血脑屏障通透性;酶联免疫吸附法(ELISA)检测脑组织中ICAM-1,VCAM-1,E-selectin表达;免疫组化法检测脑组织MMP-9表达。结果:JDTL 2,4 mL·kg-1可明显降低模型大鼠的脑组织含水量,EB含量,ICAM-1,VCAM-1,E-selectin以及MMP-9的表达(P<0.05或P<0.01)。结论:解毒通络注射液对大鼠脑缺血再灌注损伤引起的脑水肿有保护作用,其作用机制与降低模型大鼠脑组织ICAM-1,VCAM-1,E-selectin的表达、抑制脑组织MMP-9的表达有关。
Objective: To study the effect of Jiedu Tongluo injection (JDTL) on the changes of water content in brain tissue and the expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 1, E-selectin and MMP-9 in rats with cerebral ischemia-reperfusion injury. Methods: The model of middle cerebral artery occlusion (MCAO) was established by thread occlusion method. The content of water in brain tissue was measured by dry-wet weight method. The content of Evans blue (EB) The expressions of ICAM-1, VCAM-1 and E-selectin in brain tissue were detected by enzyme linked immunosorbent assay (ELISA). The expression of MMP-9 in brain tissue was detected by immunohistochemistry. Results: JDTL 2,4 mL · kg-1 could significantly reduce the water content, EB content, the expression of ICAM-1, VCAM-1, E-selectin and MMP-9 in brain tissue of model rats (P < 0.01). Conclusion: Jiedu Tongluo injection has a protective effect on cerebral edema induced by cerebral ischemia-reperfusion injury in rats. Its mechanism of action is to reduce the expression of ICAM-1, VCAM-1 and E-selectin in the brain of model rats, Tissue MMP-9 expression related.