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本研究应用ＩＨＡ、ＩＦＡＴ和ＥＬＩＳＡ法诊断技术，对采自本站日常门诊１３０份人血清进行弓形虫抗体检测，对其敏感性和特异性等指标进行比较，结果是ＩＨＡ、ＩＦＡＴ和ＥＬＩＳＡ的弓形虫抗体阳性率分别为６．９２％（９／１３０）．８．４６％（１１／１３０）和１５．３８％（２０／１３０）。３种试验方法以ＥＬＩＳＡ较敏感和特异，总符合率为８２．３１％。３种方法阳性率（％）之比为１００．００．１２２．２５：２２２．２５。ＥＬＩＳＡ法的稳定性，重复性和特异性优于ＩＨＡ法，而且操作简便、反应快速，不需特殊仪器设备，还可目测结果，试验成本低廉，便于推广和应用。
In this study, we used IHA, IFAT and ELISA to diagnose 130 human sera from daily outpatients, and compared their sensitivity and specificity with the results of IHA, IFAT and ELISA The positive rates of worm antibodies were 6.92% (9/130) respectively. 8.46% (11/130) and 15.38% (20/130). Three kinds of test methods were more sensitive and specific ELISA, the total coincidence rate was 82.31%. The positive rate (%) of the three methods was 100.00.122.25:222.25. The stability, repeatability and specificity of ELISA are superior to those of IHA. The method is simple, rapid, and does not require special equipment. It can also visualize the results, test the low cost and facilitate the promotion and application.