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溴麝香草酚兰(BTB)比色法检测胆硷脂酶活力,对有机磷中毒诊断和疗效观察有重要意义。我们在 BTB 原比色法的基础上不用酸度计和标准色列管,从试剂配制和操作方法方面进行了一些改进,方法简便易行可靠,能反映中毒程度及治疗效果。我院自1983～1987年共抢救有机磷中毒31例,应用本改进方法检测临床效果满意。试剂配制:1、0.2%(W/V)氯化乙酰胆硷(或溴化乙酰胆硷):称取氯化乙酰胆硷100mg,溶解于50ml 蒸溜水中。配成后及每次测定时试其酸度:取此液0.5ml,加蒸溜水0.1
Bromothymol blue (BTB) colorimetric assay for cholinesterase activity, the diagnosis of organic phosphorus poisoning and efficacy of great significance. Based on the original colorimetric method of BTB, we did not use the acidometer and standard color tube. We made some improvements on reagent preparation and operation methods. The method is simple and reliable, and can reflect the degree of poisoning and the therapeutic effect. Our hospital from 1983 to 1987, a total of 31 cases of organophosphate poisoning, application of this improved method to detect the clinical effect is satisfactory. Reagent preparation: 1,0.2% (W / V) acetylcholine chloride (or bromide): Weigh 100mg acetyl chloride, dissolved in 50ml of distilled water. After dubbed and each test determination of its acidity: Take this solution 0.5ml, add distilled water 0.1