论文部分内容阅读
作者用聚合酶链反应技术在人类免疫缺陷病毒 ( HIV)逆转录酶 ( RT)基因两端引入新的酶切位点 ,插入质粒 p SP72 ,再将来自质粒 p TX1 0 1的 850个碱基的 Lpp- Omp A序列 (用于外膜定位 )及 lpp和 lac启动子插入 ,构建成新的重组质粒 p HART。用同样方法构建了质粒 p RT和 p RTL
The authors used polymerase chain reaction to introduce a new restriction enzyme site at both ends of the human immunodeficiency virus (HIV) reverse transcriptase (RT) gene, inserted the plasmid pSP72, and then transferred 850 nucleotides Of Lpp-Omp A sequence (for the outer membrane localization) and lpp and lac promoter inserted into a new recombinant plasmid p HART. Plasmids p RT and p RTL were constructed in the same manner