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目的:探索冬眠心肌的能量代谢特点。方法:用悬浮人红血球的KH液灌流大鼠离体等容收缩心脏,建立急性冬眠心肌模型,以心肌三磷酸腺苷、磷酸肌酸、糖原含量,以及心肌摄取乳酸的量作为代谢指标。结果:(1)冬眠30min组心肌三磷酸腺苷(ATP)、磷酸肌酸及糖原含量均明显低于平衡末组(P<005),心肌由正常情况下摄取乳酸转为释放乳酸(P<005),在冬眠90min组,以上指标与冬眠30min组无显著差异;(2)再灌注30min,心肌磷酸肌酸和糖原含量及心肌摄取乳酸的量与平衡末组相似(P>005),但心肌三磷酸腺苷含量仍较平衡末组为低(P<005)。结论:急性冬眠期间,心肌对氧及代谢底物的供/均需比例在低水平上保持平衡,保证了心肌的存活
Objective: To explore the energy metabolism of hibernating myocardium. METHODS: Isolated rat hearts were isovosed with KH solution suspended in human erythrocytes to establish an acute hibernating myocardium model. ATP, creatine phosphate, glycogen content and lactic acid content in myocardium were taken as metabolic indices. Results: (1) The levels of ATP, creatine phosphate and glycogen in myocardium were significantly lower than those in the equilibrium group (P <005) at 30 min after hibernation, and the myocardium changed from lactic acid uptake to lactic acid release under normal conditions (P < 005). In hibernation 90 min group, there was no significant difference between the above indexes and hibernation 30 min group. (2) The content of creatine phosphate and glycogen in myocardium and the amount of lactic acid uptake in myocardium were similar to those in the end-balance group at 30 min after reperfusion 05), but myocardial adenosine triphosphate content is still lower than the balance end group (P <0 05). CONCLUSIONS: During the acute hibernation period, the myocardial supply / demand ratio of oxygen and metabolic substrates is kept at a low level to maintain myocardial viability