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背景:癫痫发作后大脑海马神经元有明显受损,而癫痫后神经细胞损害有坏死和凋亡两种形式,在癫痫神经损害中起着重要作用。腺苷作为内源性神经保护递质,可以抑制兴奋性氨基酸的释放、氧自由基的产生以及一氧化氮的作用,同时还有改善脑血流以及抗惊厥作用。但有关腺苷与癫痫后细胞凋亡之间的关系尚不完全清楚。目的:观察腺苷受体激动剂2-CAdo对癫痫大鼠海马神经细胞bcl-2,Bax基因表达的影响,进一步探讨腺苷抗惊厥及脑保护的作用机制。设计:以实验动物为研究对象,完全随机对照实验研究。单位:一所油田总医院的儿科和普外科,一所大学医院儿内科。材料:实验于2002-10/2003-03在哈尔滨医科大学实验动物学部及病理教研室完成。体质量200～250g健康成年Wistar大鼠104只,雌雄各半。动物随机分为正常组8只,致痫组32只,致痫+2-CAdo组32只,致痫+生理盐水组32只。干预:按1.5mg/kg腹腔注射马桑内酯(由哈尔滨医科大学药理学部提供)建立动物癫痫模型,全部大鼠于注射后5min出现抽搐,持续时间一两分钟。致痫+2-CAdo组于马桑内酯注射前1h及抽搐后1h经尾静脉注射2-CAdo(由ICN公司提供)剂量为0.6mg/kg,致痫+生理盐水组于马桑内酯注射前1h及抽搐后1h经尾静脉注射等量的生理盐水。主要观察指标:海马CA1区bcl-2,Bax基因表达阳性细
BACKGROUND: The hippocampal neurons in the brain are obviously damaged after the epileptic seizure, while the necrosis and apoptosis of the neuronal cells after epilepsy are two forms, which play an important role in the nerve damage of epilepsy. As a neurotransmitter of endogenous neurotransmission, adenosine can inhibit the release of excitatory amino acids, the production of oxygen free radicals and the effect of nitric oxide, while improving cerebral blood flow and anticonvulsant effect. However, the relationship between adenosine and apoptosis after epilepsy is not fully understood. Objective: To observe the effects of adenosine receptor agonist 2-CAdo on the expression of bcl-2 and Bax genes in hippocampal neurons of epileptic rats and further explore the mechanism of anticonvulsant and brain protection of adenosine. Design: The experimental animals as the research object, a completely randomized controlled experimental study. Unit: a Oilfield General Hospital of Pediatrics and General Surgery, a University Hospital Department of Pediatrics. Materials: The experiment was performed at Department of Laboratory Animal Science and Pathology, Harbin Medical University from October 2002 to March 2003. Body weight 200 ~ 250g healthy adult Wistar rats 104, male and female. Animals were randomly divided into normal group 8, epileptic group 32, epilepsy + 2-CAdo group 32, epilepsy + saline group 32. Intervention: Animal models of epilepsy were established by peritoneal injection of intra-coronolactone (1.5 mg / kg) provided by Department of Pharmacology, Harbin Medical University. All rats were convulsed 5 minutes after injection for a duration of one to two minutes. The epileptogenic + 2-CAdo group was injected with 2-CAdo (provided by ICN Company) via caudal vein 1 h prior to injection of corosalone and 0.6 mg / kg 1 h after convulsions. One hour before injection and 1 h after twitch, an equal volume of saline was injected through tail vein. MAIN OUTCOME MEASURES: The expression of bcl-2 and Bax in hippocampal CA1 region was positive