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:目的 应用改良的随机引物聚合酶链反应(AP-PCR)技术,分离配对的食管癌组织和非癌食管组织中差异基因片断,并进行克隆、测序和序列分析。方法 在来自食管癌高发区的22例配对的食管癌组织和非癌食管组织中,应用AP-PCR技术测检了差异基因片段。结果 22例配对食管癌组织和非癌食管组织中,6例癌组织中有差异随机扩增片断,而相应的非癌组织缺如。其中5T差异基因片断为10kb左右,经克隆、测序和序列的同源性分析,基因文库内无同源序列。结论 5T差异基因片断是否为一个新的侯选基因或癌基因标志物,有待进一步筛查。
OBJECTIVE: To separate the differentially expressed genes in paired esophageal cancer tissues and non-cancerous esophageal tissues by using modified random primer-polymerase chain reaction (AP-PCR) and clone, sequence and sequence analysis. Methods AP-PCR was used to detect differential gene fragments in 22 paired esophageal cancer tissues and non-cancerous esophageal tissues from high incidence area of esophageal cancer. Results Among the 22 matched esophageal cancer tissues and non-cancerous esophageal tissues, there were differences in 6 cases of cancerous tissues randomly amplified, while the corresponding non-cancerous tissues were absent. Among them, the 5T differential gene fragment is about 10kb. By homology analysis of cloning, sequencing and sequence analysis, there is no homologous sequence in the gene library. Conclusion Whether the 5T differential gene fragment is a new candidate gene or oncogene marker remains to be further screened.