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目的 探索白血病抑制因子（LIF）基因在小鼠胚泡着床后有无表达。方法 采用RT-PCR技术对孕龄5d、 7d、10d、12d、和15d的小鼠胚胎（共50个，每组 10个）LIF基因表达进行检测。结果 在孕龄 5d的二个胚胎和孕龄 7d的一个胚胎中检测到LIF基因弱表达，而在孕龄10d以上的胚胎中均未发现有LIF基因表达。结论 推测LIF基因的表达在小鼠胚胎组织中受到限制。
Objective To investigate whether the LIF gene is expressed after implantation of mouse blastocyst. Methods RT-PCR was used to detect the expression of LIF gene in embryonic mice (50 in each group, 10 in each group) at 5d, 7d, 10d, 12d, and 15d gestational ages. Results The weak expression of LIF gene was detected in two embryos at 5 days of gestational age and one embryo at 7 days of gestational age. However, no LIF gene expression was found in embryos of gestational age greater than 10 days. Conclusion It is speculated that the expression of LIF gene is limited in mouse embryonic tissues.