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色谱系统由一台Waters M6000泵、u6K进样器、岛津SPD-1可变波长紫外吸收检测器(232nm、280nm)、056记录仪组成。色谱柱为μBondapakG_(18)移动相是甲醇-0.2M醋酸铵-水(70/5/25,V/V),流速1.4毫升/分。样品用甲醇溶解配为1.0mg/10ml浓度,进样5μl测定。056记录仪记录色谱曲线,峰高乘半峰宽计算峰面积,由面积归一法计算有关组分相对含量。 色谱分析结果表明,不同菌株所得产品组分之间的比例有较大差异;而即使是同一菌株,如果发酵、提炼工艺不同,所得产品组分变化也很大,特别是发酵条件可以明显影响组分情况,见图1。与进口麦迪霉素对照并
Chromatography system consists of a Waters M6000 pump, u6K injector, Shimadzu SPD-1 variable wavelength UV absorption detector (232nm, 280nm), 056 recorder. The column was μBondapakG_ (18) The mobile phase was methanol-0.2M ammonium acetate-water (70/5/25, V / V) and the flow rate was 1.4 ml / min. Samples dissolved with methanol as 1.0mg / 10ml concentration, injection of 5μl determination. 056 Recorder records the chromatograms, the peak height by the half-width peak area calculation, the area normalization method to calculate the relative content of the relevant components. The results of chromatographic analysis showed that there were great differences in the proportions of the product components obtained from different strains. However, even if the same strain was used, the composition of the obtained product varied greatly depending on the fermentation and the refining process. In particular, the fermentation conditions significantly affected the composition Sub-situation, see Figure 1. Imported with midecamycin and control