论文部分内容阅读
目的研究超大剂量甲泼尼龙(MP)对实验性变态反应性脑脊髓炎(EAE)大鼠的治疗作用,探讨超大剂量MP能否提高疗效。方法构建EAE大鼠模型,分为超大剂量组、小剂量组和模型对照组,分别予以尾静脉注射MP 100 mg/kg、25 mg/kg、等体积生理盐水;另取正常大鼠为正常对照组,给予等体积生理盐水注射。连续给药5 d,处死大鼠,采血检测血浆白细胞介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)含量。取脑、脊髓组织行HE染色及髓鞘染色。对临床评分、炎性病灶计数、髓鞘脱失评分、细胞因子含量等进行综合评价。结果超大剂量组和小剂量组大鼠经MP治疗前后临床评分差异均有统计学意义(P<0.05),组织炎性病灶数、髓鞘脱失评分及血浆IL-2、TNF-α含量均较模型对照组低。而超大剂量、小剂量组间在临床评分、炎性病灶数、髓鞘脱失评分、细胞因子含量等方面比较均无统计学差异。结论两种剂量MP均能缓解EAE症状,改善病情,但临床疗效并不与剂量呈依赖性,在一定的有效剂量范围内,超大剂量MP并不能提高疗效。
Objective To investigate the therapeutic effect of super high dose methylprednisolone (MP) on experimental allergic encephalomyelitis (EAE) rats and to explore whether the overdose MP can improve the therapeutic effect. Methods EAE rat model was established and divided into super-large dose group, low-dose group and model control group, respectively, by tail vein injection of MP 100 mg / kg, 25 mg / kg, an equal volume of normal saline; Group, given an equal volume of saline injection. The rats were sacrificed 5 days after continuous administration, blood samples were collected for determination of plasma interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α). Take brain, spinal cord tissue HE staining and myelin staining. Clinical scores, inflammatory lesions count, demyelination score, cytokine content and other comprehensive evaluation. Results There was significant difference in the clinical scores between the large dose group and the low dose group before and after MP treatment (P <0.05), the number of inflammatory lesions, the demyelination score and the levels of plasma IL-2 and TNF-α Lower than the model control group. However, there was no significant difference in clinical scores, inflammatory lesions, demyelination score, cytokine content between the ultra-high dose and low dose groups. Conclusion Both doses of MP can relieve the symptoms of EAE and improve the condition, but the clinical efficacy is not dose-dependent. In a certain effective dose range, the over-large dose of MP can not improve the therapeutic effect.