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目的:评价肝细胞肝癌患者病灶局部免疫状况。方法:在超声引导下经皮进行肝脏穿刺活检,取出的组织标本用特异性抗体免疫组织化学染色,检测病灶区域浸润的T淋巴细胞、自然杀伤细胞、巨噬细胞、B淋巴细胞及T淋巴细胞Fas-L阳性率,测量阳性细胞占单位面积百分比及阳性细胞直径,以了解病灶局部免疫细胞分布情况。结果:肝癌病灶局部有不同程度免疫细胞浸润,主要为T淋巴细胞、自然杀伤细胞和巨噬细胞,部分T淋巴细胞和自然杀伤细胞直径>10μm,一些巨噬细胞有突起,8.0%T淋巴细胞Fas-L表达阳性。免疫细胞浸润程度依次为肝癌被膜下>癌周肝组织>肝癌组织。结论:肝癌患者病灶局部有免疫活性细胞浸润,但限于癌被膜下及癌周肝组织内,癌组织内免疫活性细胞浸润程度低下。
Objective: To evaluate the local immune status of patients with hepatocellular carcinoma. Methods: Percutaneous liver biopsy was performed under the guidance of ultrasound. Tissue samples were taken out by immunohistochemical staining with specific antibodies to detect infiltrating T lymphocytes, natural killer cells, macrophages, B lymphocytes and T lymphocytes Fas-L positive rate, measuring the percentage of positive cells per unit area and positive cell diameter, in order to understand the distribution of focal immune cells. Results: There were some degree of immune cell infiltration in HCC lesions, mainly T lymphocytes, natural killer cells and macrophages. Some T lymphocytes and natural killer cells were larger than 10μm in diameter. Some macrophages had protrusions, 8.0% T lymphocytes Fas-L expression was positive. The infiltration of immune cells in order was liver cancer capsule> perivascular liver> liver cancer. CONCLUSIONS: There are immunocompetent cells infiltrating locally in the lesions of patients with HCC, but they are confined to the lesion in the cancer tissues and the surrounding liver tissues. The infiltration of immunocompetent cells in the cancerous tissues is low.