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目的：探讨融合自杀基因ｐＣＥＡｃｄ－ｔｋ在导入到肺癌细胞后，其体外旁观者效应的细胞差异性。方法：融合基因ｐＣＥＡｃｄ－ｔｋ提取后，脂质体介导的方法转染到６种肺癌细胞株（ＳＰＣＡ－１，ＧＬＣ－８２，Ａ５４９，ＳＣ，ＨＩＡＭＰ，Ｌ－７８），用ＭＴＴ法测定在细胞转染前后对前体药物５－氟胞嘧啶（５－ＦＣ）和丙氧鸟苷（ＧＣＶ）的敏感性和在体外的旁观者效应。结果：①脂质体介导的基因转染效率很低。②在６种已转染的肺癌细胞株中，有２种肺癌细胞株（ＳＰＣＡ－１，Ａ５４９）对前体药物５－ＦＣ和ＧＣＶ有较高的敏感性；③旁观者效应显示，２种细胞株（ＳＰＣＡ－１，ＳＣ）有强的体外旁观者效应，１种细胞株（Ａ５４９）有较强的旁观者效应，而另外３种细胞株（ＧＬＣ－８２，ＨＩＡＭＰ和Ｌ－７８）的旁观者效应较差。结论：不同的细胞在转染了融合自杀基因以后，其体外旁观者效应存在较大的差异。
OBJECTIVE: To investigate the cell-by-cell bystander effect of the fusion suicide gene pCEAcd-tk in lung cancer cells. Methods: Six kinds of lung cancer cell lines (SPCA-1, GLC-82, A549, SC, HIAMP and L-78) were transfected with pCEAcd- Sensitivities and in vitro bystander effects on prodrugs 5-fluorocytosine (5-FC) and ganciclovir (GCV) before and after cell transfection. Results: ① liposome-mediated gene transfection efficiency is low. ② Among the 6 kinds of lung cancer cell lines transfected, 2 kinds of lung cancer cell lines (SPCA-1, A549) had higher sensitivity to 5-FC and GCV; ③ Bystander effect showed that two kinds of lung cancer cell lines Cell lines (SPCA-1, SC) had a strong bystander effect in vitro, one of the cell lines (A549) had a strong bystander effect, while the other three cell lines (GLC-82, HIAMP and L-78) Bystander effect is poor. CONCLUSION: There are large differences in bystander effect of different cells after transfection of fusion suicide gene.