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应用酶联免疫吸附试验(ELISA)对41例青霉素过敏病人进行血清抗青霉噻唑蛋白抗体的测定。同时进行皮内试验和青霉素离子透入法的比较。41例病人中测出抗青霉噻唑蛋白特异IgE者26例。表明青霉噻唑蛋白与青霉素过敏休克之间有密切关系。IgE是人血清中含量最少的一种免疫球蛋白。应用ELISA技术测定结果表明该方法是一种特异性高、敏感、简便易于掌握的较新技术。
41 patients with penicillin allergy were tested for anti-penicillin antibody by enzyme-linked immunosorbent assay (ELISA). At the same time, intradermal test and penicillin iontophoresis were compared. Twenty-six patients with anti-penicillin-specific IgE were detected in 41 patients. Show that there is a close relationship between penicillin and penicillin allergic shock. IgE is one of the least abundant immunoglobulins in human serum. The results of ELISA assay showed that this method is a newer technique with high specificity, sensitivity, simple and easy to grasp.