本文报道小鼠胸腺上皮细胞系(MTEC_1系)的细胞学鉴定。细胞为多角形,呈上皮样排列,用抗角蛋白抗体显示细胞胞质内均含角蛋白。电镜观察可见胞质内含成束的张力细丝,相邻细胞间有桥粒结构。用抗小鼠上皮细胞单克隆抗体进行免疫组织化学染色,几乎全部MTEC_1细胞均为MTS33阳性,提示MTEC_1细胞是来源于胸腺體质的上皮样细胞。大多数MTEC_1细胞有正常小鼠染色体数(40)条。本文结果表明,MTEC_1细胞系属正常小鼠胸腺上皮细胞。 This article reports the cytological identification of the mouse thymic epithelial cell line (MTEC-1 line). Cells were polygonal, were epithelial-like arrangement, with anti-keratin antibodies showed cytoplasm containing keratin. Electron microscopy showed that the cytoplasm contained bundles of tension filaments, desmosomes between adjacent cells. Immunohistochemical staining with anti-mouse epithelial monoclonal antibodies revealed that almost all of MTEC-1 cells were MTS33-positive, suggesting that MTEC-1 cells are epithelial-like cells derived from the thymus. Most MTEC-1 cells have a normal mouse chromosome number (40). Our results show that, MTEC_1 cell line is a normal mouse thymus epithelial cells.