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目的:观察补肾方、健脾方对大鼠肾脏、股骨中维生素D受体(vitamin D receptor,VDR)表达的影响,探讨补肾、健脾不同功效的中药复方,是否对机体不同组织器官VDRmRNA的表达存在一定选择性。方法:复制切卵大鼠骨质疏松模型,随机分为假切组、模型组、补肾方组和健脾方组,术后12周开始灌胃,灌胃12周后取材。测定骨密度、血清雌二醇浓度,同时对大鼠肾脏、股骨中VDRmRNA进行半定量。结果:补肾方能提高切卵大鼠骨密度(P<0.05),健脾方能维持大鼠骨密度(P>0.05);补肾方、健脾方均维持了切卵大鼠血清雌二醇浓度水平,上调了肾脏和股骨中VDRmRNA的表达(P<0.01)。结论:补肾和健脾中药都能通过上调切卵大鼠肾脏和股骨中VDRmRNA的表达,而起到治疗骨质疏松症的作用。
Objective: To observe the effect of Bushen Fang and Jianpi Recipe on the expression of vitamin D receptor (VDR) in rat kidney and femur, and to explore whether VDR mRNA in different tissues and organs of the body is affected by Chinese herbal compound with different efficacy of tonifying the kidney and spleen. There is a certain degree of selectivity in expression. METHODS: Osteopenia models of osteotomy rats were duplicated and randomly divided into sham-cut group, model group, Bushenfang group and Jianpifang group. The rats were intragastricly administrated 12 weeks after operation and were obtained after intragastric administration for 12 weeks. Bone mineral density and serum estradiol concentration were measured, and VDR mRNA in rat kidney and femur was also semi-quantitated. RESULTS: Bushen Prescription could increase bone density in the oocytes of rats (P<0.05). Jianpi Preservation can maintain the bone density in rats (P>0.05). Both Bushenfang and Jianpi Preservation of serum estradiol in the oocyte-shearing rats At the concentration level, VDR mRNA expression in the kidney and femur was upregulated (P<0.01). Conclusion: Both Bushen and Jianpi herbs can increase the expression of VDR mRNA in the kidney and femur of the osteotomy rats and play an important role in the treatment of osteoporosis.