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过去工作表明,PAG内注入吗啡产生的镇痛作用,至少有部分需经上行至伏核方能实现。并证明在PAG-伏核镇痛信息传递中,伏核内的5-羟色胺(5-HT)和脑啡肽起重要作用。本工作对伏核内达两种神经递质的相互关系作进一步研究。用辐射热甩头法测痛。予先向家兔伏核埋植不锈钢瘘管,脑内注射容积为1μl,注射部位经脑切片鉴定。 1.伏核内注射5-HT引起的镇痛可被阿片受体阻断剂纳洛酮所对抗:22只家兔每侧伏核内注射5-HT10μg,10分钟后痛阈升高,30分钟作用达高峰。达时再经同一注射管内每侧注入纳洛酮[即(±)纳洛酮]4μg,或注入(+)纳洛酮4μg作为对照。10分钟后(+)纳洛酮组即对照组痛
Past work has shown that at least some of the analgesic effect of morphine injection into PAG can be achieved only by ascending to the nucleus accumbens. And demonstrated that serotonin (5-HT) and enkephalins in the nucleus accumbens played an important role in the transmission of analgesic information on PAG-V nucleus. In this work, the relationship between two neurotransmitters in V nucleus was further studied. With radiant heat shame to measure pain. Pre-implantation of stainless steel fistula to the nucleus accumbens, intracerebral injection volume of 1μl, the injection site identified by brain sections. 1. V-nucleus injection of 5-HT-induced analgesia can be opioid antagonist antagonist naloxone: 22 rabbits each nucleus accumbens injected 5-HT10μg, 10 minutes after the pain threshold increased 30 Minute effect reached the peak. Up to 4 μg of naloxone [ie (±) naloxone) or 4 μg of (+) naloxone was injected into each side of the same syringe as control. After 10 minutes (+) naloxone group control group pain