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应用花粉管通道技术将天蚕抗菌肽Ｂ基因导入烟草子房，经多世代接种青枯菌鉴定，并经胭脂碱、点杂交、ＰＣＲ扩增和Ｓｏｕｔｈｅｒｎｂｌｏｔ检测。结果表明，抗菌肽Ｂ基因已整合到烟草基因组并传递到后代中。田间发病率和病情指数分析显示Ｔ３，Ｔ４代转基因株系抗青枯病能力有明显提高。
The pollen tube pathway was used to transfer the Bombyx mori B gene into tobacco ovary and identified by R. solanacearum for many generations. The results were confirmed by nopaline, spot blot, PCR and Southern blot. The results showed that the antimicrobial peptide B gene has been integrated into the tobacco genome and passed into the offspring. Field incidence and disease index analysis showed that T3 and T4 generation of transgenic lines have significantly improved resistance to bacterial wilt.