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目的探讨类表皮生长因子受体2(human epidermalgrowth factor receptor 2,HER2)蛋白表达及基因扩增与胃癌临床病理特征的关系。方法分别用免疫组织化学方法和荧光原位杂交方法检测60例胃癌与癌旁非肿瘤胃黏膜中HER2蛋白表达和基因扩增状态,分析HER2蛋白表达及基因扩增与胃癌各临床病理特征的相关性。采用SPSS 16.0统计软件对数据进行x~2检验分析,P<0.05为差异有统计学意义。结果 60例癌旁非肿瘤胃黏膜中无HER2蛋白表达及基因扩增。胃癌组织中HRE2蛋白过表达率及基因扩增率分别为16.67%(10/60)、23.33%(14/60)。HRE2蛋白过表达及基因扩增与胃癌发病年龄、组织学分型、浸润深度及淋巴结转移相关(均P<0.05)。结论 HER2可作为判断胃癌生物学行为和预后的参考指标。
Objective To investigate the relationship between the expression of HER2 protein and gene amplification and the clinicopathological features of gastric cancer. Methods Immunohistochemistry and fluorescence in situ hybridization were used to detect HER2 protein expression and gene amplification in 60 cases of gastric cancer and adjacent non-tumor gastric mucosa respectively. The correlation between HER2 protein expression and clinicopathological characteristics of gastric cancer was analyzed Sex. Using SPSS 16.0 statistical software for data x ~ 2 test analysis, P <0.05 for the difference was statistically significant. Results There were no HER2 protein expression and gene amplification in 60 cases of adjacent non-tumor gastric mucosa. The overexpression rate and gene amplification rate of HRE2 in gastric cancer tissues were 16.67% (10/60) and 23.33% (14/60), respectively. HRE2 overexpression and gene amplification correlated with the age of onset, histological type, depth of invasion and lymph node metastasis (all P <0.05). Conclusion HER2 can be used as a reference index to judge the biological behavior and prognosis of gastric cancer.