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目的探讨短暂脑缺血发作(TIA)患者血清内源性硫化氢(H2S)表达水平及胱硫醚-γ-合酶(CBS)活性的变化及临床意义。方法采集60名TIA患者空腹静脉血标本,采用敏感硫电极法测定H2S血清浓度,采用Elisa试剂盒测定CBS活性,与30例健康成年人所测结果进行比较,并对不同预后的患者血清H2S表达水平及CBS活性进行对比分析。结果健康成人外周血内源性H2S表达水平及CBS活性低于TIA患者(P<0.05),在TAT患者中,进展为脑梗死患者外周血内源性H2S表达水平及CBS活性高于未进展为脑梗死的患者(P<0.05),差异具有统计学意义。结论TIA患者早期即可出现内源性H2S表达水平异常,CBS活性改变,内源性H2S较早地预测、估计TIA演变趋势,对估计病情,预测预后具有重要的临床意义。
Objective To investigate the changes and clinical significance of serum H2S level and cystathionine-γ-synthase (CBS) activity in patients with transient ischemic attack (TIA). Methods Fasting venous blood samples were collected from 60 patients with TIA. The concentrations of H2S in serum were determined by the sensitive sulfur electrode method. The activity of CBS was measured by Elisa kit and the results were compared with those in 30 healthy adults. The serum H2S expression in patients with different prognosis Level and CBS activity comparative analysis. Results The expression of endogenous H2S and the activity of CBS in peripheral blood of healthy adults were lower than those of TIA patients (P <0.05). In patients with TAT, the expression of endogenous H2S and the activity of CBS in patients with cerebral infarction were higher than those without T Patients with cerebral infarction (P <0.05), the difference was statistically significant. Conclusions The abnormal expression of endogenous H2S, the change of CBS activity and the earlier prediction of endogenous H2S in early TIA patients are estimated. It is of great clinical significance to estimate the prognosis and prognosis of TIA patients.