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目的研究肺缺血再灌注(ischemia/reperfusion,I/R)对乙醛脱氢酶2(ALDH2)活性和含量,丙二醛(Methane Dicarboxylic Aldehyde,MDA)含量及4羟壬烯醛(4-hydroxy-2-nonenal,4-HNE)含量的影响。方法选取36只250-350g的雄性SD大鼠,采用在体原位肺I/R损伤模型,根据缺血再灌注时间不同随机分为A组(空白对照组即sham组)、B组(平衡灌注15min组)、C组(缺血停呼吸30min组)、D组(缺血停呼吸60min组)、E组(再灌注恢复呼吸30min组)和F组(再灌注恢复呼吸60min组),每组6只。在观察结束后取肺组织检测ALDH2活性和含量,MDA和4-HNE含量。结果 A组、B组、C组、D组、E组和F组ALDH2活性分别为(2.26±0.45,2.30±0.48,2.40±0.69,2.21±0.42,2.21±0.42和2.16±0.62),含量分别为(0.91±0.11,1.21±0.61,1.23±0.38,1.11±0.38,1.00±0.47和0.92±0.34)组间差异无统计学意义。D组、E组和F组大鼠肺MDA含量(11.03±0.51,12.10±0.29和11.73±0.57)较A组和B组(10.35±0.52和10.30±0.56)升高,差异有统计学意义(P<0.05);E组和F组大鼠肺MDA含量高于C组(10.87±0.62)和D组,差异有统计学意义(P<0.05);其他各组之间差异无统计学意义。D组、E组和F组大鼠肺4-HNE含量(2.15±0.09,2.81±0.22和2.76±0.31)较A组和B组(1.93±0.11和1.97±0.10)升高,差异有统计学意义(P<0.05);E组和F组大鼠肺4-HNE含量高于C组(2.05±0.08)和D组,差异有统计学意义(P<0.05);其他各组之间差异无统计学意义。结论随着缺血时间的延长,肺中醛类物质(MDA和4-HNE)开始蓄积,再灌注过程中醛类物质继续升高,ALDH2活性和含量未受到明显抑制
Objective To investigate the effect of I / R on the activity and content of aldehyde dehydrogenase 2 (ALDH2), the content of malondialdehyde (MDA) and the content of 4- hydroxynonenal (4- hydroxy-2-nonenal, 4-HNE) content. Methods Thirty-six male Sprague-Dawley rats weighing 250-350 g were randomly divided into group A (blank control group, sham group) and group B (balanced Group C (ischemic resuscitation 30min), group D (60min resuscitation), group E (resuscitation resuscitation 30min) and group F (resuscitation resuscitation 60min) Group of six. After the observation, the lung tissues were taken to measure the activity and content of ALDH2, MDA and 4-HNE content. Results The ALDH2 activities in groups A, B, C, D, E and F were (2.26 ± 0.45, 2.30 ± 0.48, 2.40 ± 0.69, 2.21 ± 0.42, 2.21 ± 0.42 and 2.16 ± 0.62, (0.91 ± 0.11, 1.21 ± 0.61, 1.23 ± 0.38, 1.11 ± 0.38, 1.00 ± 0.47 and 0.92 ± 0.34 respectively). There was no significant difference between the two groups. The content of MDA in lungs in groups D, E and F were significantly higher than that in groups A and B (10.35 ± 0.52 and 10.30 ± 0.56) (11.03 ± 0.51,12.10 ± 0.29 and 11.73 ± 0.57, respectively) P <0.05). The contents of MDA in lungs of rats in group E and group F were significantly higher than those in group C (10.87 ± 0.62) and group D (P <0.05). There was no significant difference among the other groups. The levels of 4-HNE (2.15 ± 0.09, 2.81 ± 0.22 and 2.76 ± 0.31) in groups D, E and F were significantly higher than those in groups A and B (1.93 ± 0.11 and 1.97 ± 0.10) (P <0.05). The levels of 4-HNE in lung tissue of rats in E group and F group were significantly higher than those in C group (2.05 ± 0.08) and D group (P <0.05). There was no significant difference between the other groups Statistical significance. Conclusions Aldehydes (MDA and 4-HNE) in the lung began to accumulate with the prolongation of ischemic time. The aldehydes continued to increase and the activity and content of ALDH2 were not significantly inhibited during reperfusion