本实验旨在探讨高脂饮食下动脉粥样硬化微血管—特别是内皮细胞的病理改变及抗氧化维生素E、维生素c和β-胡萝卜素伍用的保护作用。用19只成年日本大耳白兔(雄性,体重2.5～3.0 kg,中国农科院实验动物中心提供),制备透明活体微血管及微循环耳窗模型。动物分为3组:正常对照组5只,喂正常饮食,不给任何药物;高脂饮食组5只,喂高脂饮食(每只每日1.5 g胆固醇,购自广州化学试剂玻璃仪器批发部;2.5克猪油,余正常饮食);高脂抗氧化维生素组9只,喂高脂饮食(同上)和抗氧化维生素(每只每日维生素E 100 mg,维生素C 200 mg,均购自北京制药厂;β-胡萝卜素5 mg,瑞士罗氏药品化学有限公司提供)。通过耳窗,借助显微电视录相系统(Olympus-A型显微镜,Sony彩色摄像机,Sharp-789型录像机,Sony显示器)观察、记录上述各组动物实验前及实验后每周、连续12周的外周微血管内皮细胞的情况。结果:(1)高脂饮食下,微血管内皮 The purpose of this study was to investigate the pathological changes of atherosclerotic microvessels, especially endothelial cells, and the protective effect of antioxidants such as vitamin E, vitamin C and β-carotene on high fat diet. Nineteen adult Japanese white rabbits (male, weighing 2.5-3.0 kg, provided by Experimental Animal Center of Chinese Academy of Agricultural Sciences) were used to prepare transparent live microvascular and microcirculation ear window models. The animals were divided into 3 groups: normal control group 5, fed normal diet, no drugs; high-fat diet group 5, fed a high-fat diet (each 1.5 g cholesterol per day, ; 2.5g lard, the rest of the normal diet); high-fat antioxidant vitamins group 9, fed a high-fat diet (ibid.) And antioxidant vitamins (daily vitamin E 100 mg, vitamin C 200 mg were purchased from Beijing Pharmaceutical Factory; β-carotene 5 mg, provided by Roche Pharmaceuticals Switzerland). Through the ear window, observed and recorded by means of a video recording system (Olympus-A microscope, Sony color video camera, Sharp-789 video recorder, Sony monitor), the animals in each group were recorded before and after the experiment for 12 weeks Peripheral microvascular endothelial cells. Results: (1) Under high fat diet, microvascular endothelium