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目的 探讨在实验性变态反应性神经炎 (EAN)中特异性识别抗原并呈优势扩增的为哪些Vβ亚家族基因。 方法 应用逆转录 聚合酶链反应 (RT PCR)、原位杂交技术 ,比较T细胞受体(TCR)Vβ2、6、8、10、14基因mRNA在EAN组和对照组大鼠外周血、淋巴结及周围神经组织中的表达水平。结果 (1)TCRVβ6、8基因mRNA在EAN大鼠淋巴结中的表达 (个 HP ,下同 )早期即有升高 (分别为 41 1± 1 1和 74 4± 1 9,对照组为 2 5 9± 1 5和 2 6 1± 1 6 ) ,至发病高峰期更加明显 ,恢复期则与对照组无显著差异。 (2 )EAN组大鼠周围神经浸润T淋巴细胞的TCRVβ 6、8基因mRNA的表达从潜伏期 (分别为 4 9± 2 9和 11 7± 9 1)至发病高峰期 (分别为 18 8± 5 8和 36 5± 6 3)逐步增加 ,恢复期又有所下降 ,差异有极显著意义。 (3)EAN大鼠发病早期及高峰期淋巴结与周围神经浸润淋巴细胞中TCRVβ 8基因mRNA的表达高于TCRVβ 6基因 ,差异有极显著意义。 结论 T细胞上识别和限制EAN发病的特异性抗原的TCRVβ基因亚家族为TCRVβ 6和Vβ 8,以Vβ 8为主 ;特异性表达TCRVβ 6、8基因的T淋巴细胞在淋巴结中被激活并克隆性扩增 ,继而迁移至病变的周围神经组织中 ,可能导致一系列的免疫损害。
Objective To investigate which Vβ subfamily genes are predominantly expressed in the experimental allergic neuritis (EAN). Methods Reverse transcriptase polymerase chain reaction (RT PCR) and in situ hybridization were used to compare the expression of Vβ2, 6, 8, 10 and 14 mRNA in peripheral blood, lymph nodes and lymph nodes of EAN group and control group Peripheral nervous tissue expression levels. Results (1) The expression of TCRVβ6,8 mRNA in the lymph nodes of EAN rats (a HP, the same below) were elevated at the early stage (41 1 ± 1 1 and 74 4 ± 1 9, respectively, while the control group was 259 ± 1 5 and 2 6 1 ± 1 6) to the peak of the onset of the more obvious recovery and no significant difference with the control group. (2) The mRNA expression of TCRVβ 6,8 in peripheral blood infiltrating T lymphocytes in EAN group increased from the latent period (49 ± 29 and 11 7 ± 9 1 respectively) to the peak incidence (18 8 ± 5 8 and 36 5 ± 6 3) gradually increased, the recovery period has declined, the difference is extremely significant. (3) The expression of TCRVβ 8 mRNA in EAN rats was higher than that in TCRVβ 6 in early stage and peak lymph nodes and peripheral infiltrating lymphocytes. The difference was significant. Conclusion The TCRVβ gene subfamilies on T cells that recognize and restrict the development of EAN are TCRVβ 6 and Vβ 8, and Vβ 8 is the major component. T lymphocytes that express TCRVβ 6 and 8 genes specifically are activated in the lymph nodes and cloned Sexual amplification, and subsequent migration to diseased peripheral nerve tissue, can result in a range of immune damage.