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目的研究低温等离子体在灭菌过程中过氧化氢气体扩散和等离子放电杀菌效果,探讨灭菌过程的主导因素。方法采用载体定量杀菌试验方法,分别观察过氧化氢低温等离子体灭菌过程中过氧化氢扩散和等离子体放电的杀菌效果。结果在过氧化氢低温等离子体灭菌装置设定条件下,灭菌腔内温度50℃、过氧化氢扩散2.5 mg/L,不启动放电过程,作用42 s可完全杀灭载体上嗜热脂肪肝菌芽孢。在灭菌腔内温度50℃条件下,不用过氧化氢气体,仅启动等离子放电作用15 min,对载体上嗜热脂肪肝菌芽孢杀灭对数值<2.0。结论在过氧化氢低温等离子体灭菌过程中,其主导杀菌作用是气化过氧化氢及其扩散状态,等离子放电仅起到辅助作用。
Objective To study the bactericidal effect of hydrogen peroxide gas diffusion and plasma discharge in the process of sterilization of low temperature plasma and to discuss the dominant factors of the sterilization process. Methods The quantitative bactericidal test was used to observe the bactericidal effect of hydrogen peroxide diffusion and plasma discharge during the hydrogen peroxide plasma sterilization process. Results Under the setting conditions of hydrogen peroxide plasma sterilizer, the temperature in the sterilization chamber was 50 ℃ and the diffusion of hydrogen peroxide was 2.5 mg / L, the discharge process was not started, and the action of 42 s could completely kill the thermophilic fat on the carrier Liver spores. In the sterilization chamber temperature of 50 ℃ conditions, without hydrogen peroxide gas, only start the plasma discharge for 15 min, on the carrier of heat-killed fatty liver spores kill log <2.0. Conclusion In the hydrogen peroxide hydrogen plasma sterilization process, its dominant bactericidal effect is the vaporization of hydrogen peroxide and its diffusion state, plasma discharge only play a supporting role.