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目的观察利用微流控免疫磁珠分选仪富集外周血循环肿瘤细胞(circulating tumor cells,CTCs)的效果。方法采用Iso Flux系统(微流体免疫磁珠分选系统),对16例肺癌患者(TMN分期法,Ⅰ~Ⅳ各4例患者)外周血中循环肿瘤细胞进行分离富集,并通过免疫荧光的方法标记CTCs的标记蛋白,对分选出的CTCs细胞进行了鉴定。结果 16位肺癌患者血样中10位患者的血样中均发现了Cytokeratine阳性和CD45阴性的CTCs,Ⅰ~Ⅳ期各4例患者的血样中检出CTCs细胞检出率分别为50%、50%、75%和100%。结论利用微流控免疫磁珠分选仪可以有效地富集外周血中稀有的循环肿瘤细胞,具有较高的灵敏性及特异性。
Objective To observe the effect of enrichment of peripheral circulating tumor cell (CTCs) by microfluidic immunomagnetic separation device. Methods Iso Flux system (microfluidic immunomagnetic beads sorting system) was used to isolate and enrich peripheral blood circulating tumor cells from 16 patients with lung cancer (TMN staging, 4 to 4 patients each) and passed immunofluorescence. Methods The labeled proteins of CTCs were labeled, and the CTCs cells sorted out were identified. Results Cytokeratine-positive and CD45-negative CTCs were found in blood samples from 10 patients in 16 patients with lung cancer. The detection rates of CTCs in blood samples from 4 patients in each phase I to IV were 50% and 50%, respectively. 75% and 100%. Conclusion The use of microfluidic immunomagnetic separation device can effectively enrich rare circulating tumor cells in peripheral blood, which has high sensitivity and specificity.