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目的:研究玄参种质资源的遗传多样性及亲缘关系。方法:应用目标起始密码子多态性(SCoT)技术对来源于全国各地的48份玄参种质进行遗传多态性分析。遗传距离采用TREECONW软件计算,UPGMA方法构建亲缘关系树状图。结果:28条引物共检测到279个位点,其中214个为多态位点,占76.7%。遗传距离变化范围在0.150 7～0.493 3。聚类结果显示栽培玄参亲缘关系较为复杂:有的种质具明显的地理相关性;有的种质聚类混杂,与地理分布无明显相关性;也有来源于同一地区的种质仅部分聚在一起,呈现出一定的地域性分布规律。结论:栽培玄参的遗传多样性水平较高,为优良品种的培育提供了丰富的遗传基础。
Objective: To study the genetic diversity and genetic relationship of the figwort resources. Methods: The genetic diversity of 48 Chinese figwort (Genna xanthoxylum) germplasm collected from all over China was analyzed by using the target start codon polymorphism (SCoT). Genetic distance using TREECONW software calculation, UPGMA method to construct the relationship tree. Results: A total of 279 loci were detected by 28 primers, of which 214 were polymorphic, accounting for 76.7%. The genetic distance ranged from 0.150 7 to 0.493 3. The clustering results showed that the genetic relationship of Scrophulariaceae was complicated: some germplasm had obvious geographical correlation; some of the germplasm were mixed and had no obvious correlation with geographical distribution; and some germplasm from the same area only partially clustered Together, showing a certain geographical distribution. Conclusion: The genetic diversity of Scrophularia ningpoensis is high, which provides a rich genetic basis for breeding elite varieties.