本文不经分离用反相高效液相色谱法直接测定含芦丁的原生药及中成药中芦丁含量,并且考察了芦丁提取生产工艺。用75%甲醇水浴回流90min,采用Waters u-Bondapak-ODS柱,以甲醇∶水∶醋酸(55∶40∶5)为流动相,淫羊霍甙为内标物,直接进样,于359nm处检测,同时采用外标法,直接测定芦丁含量,并对精密度、回收率、容量因子等作了较详细的考察,本法灵敏、简便、准确。 In this paper, the rutin content in rutin-containing proto-drugs and proprietary Chinese medicines was directly determined by reversed-phase high-performance liquid chromatography without separation, and the extraction process of rutin was investigated. After refluxing in a 75% methanol water bath for 90 min, a Waters u-Bondapak-ODS column was used with methanol:water:acetic acid (55:40:5) as the mobile phase. Epimedium was used as the internal standard and injected directly at 359 nm. Detection, while using external standard method, direct determination of rutin content, and precision, recovery rate, capacity factor made a more detailed inspection, this method is sensitive, simple and accurate.