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目的:检测慢性浅表性胃炎和十二指肠球部溃疡患者外周血T淋巴细胞亚群水平的变化及炎性细胞因子白细胞介素-6(IL-6)和白细胞介素-8(IL-8)的血清含量,分析其与幽门螺杆菌(Hp)的关系,探讨可能的发病机制。方法:收集91例经内镜检查证实慢性浅表性胃炎和十二指肠球部溃疡患者的血清标本,用流式细胞仪测T淋巴细胞亚群值,用酶联免疫吸附法(ELISA)检测IL-6和IL-8含量,并分析其与Hp的关系。结果:慢性浅表性胃炎和十二指肠球部溃疡患者外周血CD8及CD4/CD8与正常对照组比较差异有显著性(P<0.01)。血清IL-6含量与正常对照组比较差异有显著性(P<0.01)。血清IL-8含量与正常对照组比较差异有显著性(P<0.01)。Hp阳性者IL-6含量低于Hp阴性者(P<0.01)。Hp阳性者IL-8含量高于Hp阴性者。结论:慢性浅表性胃炎和十二指肠球部溃疡患者CD8细胞数较正常人显著升高,CD4/CD8比值较正常人显著降低。血清IL-6、IL-8含量明显增高。Hp阳性者IL-8含量增高而IL-6含量下降。
Objective: To detect the levels of T lymphocyte subsets in peripheral blood of patients with chronic superficial gastritis and duodenal ulcer and the levels of inflammatory cytokines interleukin-6 (IL-6) and interleukin-8 -8) serum levels, analysis of its relationship with Helicobacter pylori (Hp), to explore the possible pathogenesis. Methods: Totally 91 serum samples from patients with chronic superficial gastritis and duodenal ulcer were collected by endoscopy. The T lymphocyte subsets were detected by flow cytometry (FCM) The levels of IL-6 and IL-8 were detected and their relationship with Hp was analyzed. Results: The levels of CD8 and CD4 / CD8 in peripheral blood of patients with chronic superficial gastritis and duodenal ulcer were significantly different from those of normal control group (P <0.01). Serum IL-6 levels compared with the normal control group, the difference was significant (P <0.01). Serum IL-8 levels compared with the normal control group, the difference was significant (P <0.01). Hp-positive IL-6 levels were lower than Hp-negative (P <0.01). Hp-positive patients with higher levels of IL-8 than Hp-negative. Conclusion: The number of CD8 cells in patients with chronic superficial gastritis and duodenal ulcer was significantly higher than that in normal subjects, and the ratio of CD4 / CD8 was significantly lower than that in normal subjects. Serum IL-6, IL-8 levels were significantly higher. Hp-positive IL-8 content increased IL-6 content decreased.