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用胃镜的活检组织放射自显影,研究了84例胃粘膜细胞增生水平,与癌前病变严重性的关系。用细胞增殖水平、有丝分裂指数和H~3标记率作为胃上皮恶性变的主要标准。 取出的胃粘膜组织在加有5μci/ml[~3H]-thym-idline培养液中37℃培养1小时后,Carnoy’s固定,制成石蜡切片。切片用“M”型光敏化剂复盖并在4℃曝露40天,用铁苏木精和曙红染色。在放射自显影片上计算800～1,200个细胞的标记上的细胞核数,然后计算标记指数(LI)。用同样的细胞数计算细胞的有丝分裂数和有丝分裂指数(MI)。
The biopsy tissue of the gastroscope was autoradiographed to study the relationship between the proliferation of gastric mucosa and the severity of precancerous lesions in 84 cases. Using cell proliferation levels, mitotic index and H~3 labeling rate as the main criteria for malignant transformation of gastric epithelium. The removed gastric mucosal tissue was cultured in a 5 μg/ml [~3H]-thym-idline medium at 37°C for 1 hour, and Carnoy’s was fixed to prepare a paraffin section. Sections were covered with “M” type photosensitizer and exposed for 40 days at 4°C and stained with iron hematoxylin and eosin. The number of nuclei on the label of 800 to 1,200 cells was calculated on an autoradiogram and the labeling index (LI) was calculated. The same number of cells was used to calculate the number of cells for mitosis and the mitotic index (MI).