论文部分内容阅读
目的探讨儿童感染肺炎链球菌(streptococcus pneumoniae,SPN)对常用抗菌药物的耐药性及其耐药基因分布。方法选择2013年1月—2015年12月收治的206例感染SPN患儿,进行抗生素药敏试验并应用聚合酶链反应技术检测SPN耐药基因。计数资料比较均采用Z检验,P<0.05为差异有统计学意义。结果 SPN对红霉素的耐药率最高,耐药率为95.6%;对四环素和克林霉素的耐药率分别为84.5%、87.4%;所有菌株对万古霉素和利奈唑胺敏感。197株红霉素耐药株中,耐药基因以携带ermB基因84.8%为主,携带mefE基因和mefA基因分别为44.7%、6.1%,其中22.8%携带ermB基因和mefE基因,5.1%携带ermB基因和mefA基因。结论儿童感染的SPN对大环内酯类抗生素耐药率较高;耐药基因以ermB基因介导的靶位改变为最常见,ermB基因、mefE基因和mefA基因三者单独或共同表达均可致SPN红霉素耐药。
Objective To investigate the drug resistance and drug resistance gene distribution of common antibacterials in children infected with streptococcus pneumoniae (SPN). Methods From January 2013 to December 2015, 206 children with SPN were selected for antibiotic susceptibility testing and polymerase chain reaction (PCR) was used to detect SPN resistance gene. Counting data were compared using the Z test, P <0.05 for the difference was statistically significant. Results The drug resistance rate of SPN to erythromycin was the highest, the rate of drug resistance was 95.6%. The resistance rates to tetracycline and clindamycin were 84.5% and 87.4%, respectively. All the strains were sensitive to vancomycin and linezolid. Of the 197 strains of erythromycin-resistant cells, 84.8% of the genes carrying ermB gene were dominant and 44.7% and 6.1% of those carrying mefE gene and mefA gene, respectively, of which 22.8% carried ermB gene and mefE gene and 5.1% carried ermB gene Gene and mefA gene. CONCLUSIONS: The infection rate of SPN in children is higher than that of macrolide antibiotics. The resistance genes are most frequently changed by ermB gene. The expression of ermB gene, mefE gene and mefA gene can be expressed separately or co-expressed To SPN erythromycin resistance.