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目的 研究溃疡性结肠炎 (UC)患者的人类白细胞抗原 DR(HLA DR)基因分型 ,并分析其与抗中性粒细胞胞浆抗体 (ANCA)及疾病分型的关系。方法 用间接免疫荧光法分别对 80例UC患者和 12 3例健康者进行血清ANCA检测 ,同时用序列特异性引物 聚合酶链反应法 (PCR SSP)对HLA DR第 1到第 4个基因位点进行分析。结果 UC患者ANCA的阳性率为 5 5 .0 % ,对照组均为阴性 ;UC患者HLA DR2及DR15基因阳性率分别为 5 8.8%和 40 .0 % ,较对照组 30 .1%、17.9%显著增高 (P均<0 .0 5 ) ;与ANCA(- )UC患者比较 ,ANCA(+ )UC的DR15基因阳性率显著增加 ,而DR16基因阳性率显著降低 ;慢性持续型UCDR2和DR15基因阳性率较其他型显著增加。结论 HLA DR2和ANCA在UC中阳性率均显著增加 ,在ANCA阳性和阴性的UC中 ,分别由DR15和DR16基因阳性率增加所致 ;HLA DR基因分型与临床分型有关。
Objective To study HLA DR genotyping in patients with ulcerative colitis (UC) and to analyze its relationship with antineutrophil cytoplasmic antibodies (ANCA) and disease typing. Methods Serum ANCA was detected in 80 patients with UC and 123 healthy controls by indirect immunofluorescence method. The sequences of the first to the fourth gene loci of HLA DR were detected by polymerase chain reaction (PCR) Analyze. Results The positive rate of ANCA in UC patients was 55.0%, which was negative in control group. The positive rates of HLA DR2 and DR15 gene in UC patients were 58.8% and 40.0% respectively, which were 30.1% and 17.9% (P <0.05). Compared with ANCA (-) UC patients, the positive rate of DR15 gene in ANCA (+) UC group was significantly increased, while the positive rate of DR16 gene was significantly decreased. The positive rate of chronic persistent UCDR2 and DR15 genes Compared with other types of significantly increased. Conclusions The positive rates of HLA DR2 and ANCA in UC are significantly increased. The positive rates of DR15 and DR16 are respectively increased in ANCA positive and negative UC patients. HLA DR genotyping is associated with clinical classification.