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目的探讨白细胞介素-17A(IL-17A)、白细胞介素-17F(IL-17F)基因多态性与接尘工人肺部炎症遗传易感性的相关性。方法选择67例肺部炎症患者作为病例组,126例相同粉尘暴露的健康工人作为对照组,现场监测其粉尘暴露情况;调查其人口学特征、职业信息等;采集外周静脉血,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术分析和验证IL-17A(G-197A)和IL-17F(7488T/C)两位点基因多态性,对IL-17A、IL-17F基因多态性与接尘工人肺部炎症易感性进行logistic回归分析。结果 1193例接尘工人中IL-17A(G-197A)基因型A/A、A/G、G/G分别有42例(21.76%)、95例(49.22%)、56例(29.02%);IL-17F(7488T/C)基因型T/T、T/C、C/C分别有128例(66.32%)、54例(28.98%)、11例(5.70%);各基因型的分布频率符合遗传学的HardyWeinberg平衡。2病例组IL-17A(G-197A)A/A基因型比例低于对照组(P<0.05),而A/G、G/G基因型比例均高于对照组(P<0.05);经年龄、吸烟情况、接尘浓度调整后,IL-17A(G-197A)A/G基因型(OR=5.03,P<0.01)、G/G基因型(OR=3.35,P<0.05)是接尘工人患肺部炎症的易感因素。3IL-17F(7488T/C)各基因型在病例组、对照组间分布差异无统计学意义。结论在相同的粉尘暴露条件下,与IL-17A(G-197A)A/A基因型相比,A/G、G/G基因型个体具有更高的患肺部炎症的风险,提示IL-17A(G-197A)A/G、G/G基因型的接尘个体可能对肺部炎症更具有易感性。
Objective To investigate the association between interleukin-17A (IL-17A), interleukin-17F (IL-17F) gene polymorphism and susceptibility to lung inflammation in workers exposed to dust. Methods Sixty-seven patients with lung inflammation were selected as the case group and 126 healthy workers exposed to the same dust as the control group. The dust exposure was monitored on the spot. Demographic characteristics and occupational information were also collected. Peripheral venous blood was collected and the polymerase chain The gene polymorphisms of IL-17A (G-197A) and IL-17F (7488T / C) were analyzed and verified by PCR-RFLP. 17F gene polymorphism and dust workers lung inflammation susceptibility logistic regression analysis. Results There were 42 cases (21.76%), 95 cases (49.22%) and 56 cases (29.02%) of IL-17A genotypes of A / G, G / 128 cases (66.32%), 54 cases (28.98%) and 11 cases (5.70%) of IL-17F (7488T / C) genotype T / T, T / C and C / Frequency consistent with the Hardy Weinberg equilibrium of genetics. The ratio of A / G genotype of IL-17A (G-197A) in 2 cases was lower than that in control group (P <0.05), while the ratio of A / G and G / G genotypes was higher than that of control group The genotypes of IL-17A (G-197A) A / G (OR = 5.03, P <0.01) and G / G genotype (OR = 3.35, P <0.05) after adjusting for age, Dust workers suffering from lung inflammation susceptibility factors. There was no significant difference in the distribution of 3IL-17F (7488T / C) genotype between case group and control group. Conclusions Individuals with A / G and G / G genotypes have a higher risk of lung inflammation than IL-17A (G-197A) A / A genotypes under the same dust exposure conditions, suggesting that IL- Dust-bearing individuals with 17A (G-197A) A / G and G / G genotypes may be more susceptible to pulmonary inflammation.