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将溶组织内阿米巴(HJ-2:KEIO株)和结肠内阿米巴(Olivia Ⅱ株)分别置于螺旋盖培养管内,在35℃中培养72小时,经收集、洗涤后,再悬浮于培养基中,使其每毫升含有2.5×10~5原虫滋养体。每个含5ml培养液的培养管内移入0.1ml的悬液,离心后移出
Entamoeba histolytica (HJ-2: KEIO strain) and Entamoeba histolytica (Olivia Ⅱ strain) were placed in a screw cap culture tube and cultured at 35 ° C for 72 hours. After collected, washed and resuspended In the medium, it contains 2.5 × 10 5 protozoal trophozoites per ml. Each containing 5ml culture medium into the culture tube 0.1ml suspension, centrifuged and removed