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目的制备具有稳定磁性、能够携带化疗药物的半乳糖化白蛋白磁性纳米粒,并对纳米粒的磁性、药物含量、包封率、粒径大小等物理性质进行检测。方法运用正交试验设计方法设计实验,以粒径大小、纳米药物的载药量和包封率3个指标考察固化温度、固化时间、搅拌速度及糖化比率四个因素的四个水平,从中选出最优化组合,并以最优化组合重复实验,验证试验条件的稳定性以及实验的可重复性。先行制备半乳糖化白蛋白,将阿霉素、半乳糖化白蛋白、磁粉按一定比例混合,通过在精制棉仔油中超声乳化、加热变性固化、乙醚洗涤等工艺制作出载药纳米粒,用乙醇提取法提取纳米粒中的阿霉素,并用紫外分光光度计测定含量,激光粒度分析仪分析粒径大小。结果运用最优化条件制备的纳米粒,其平均粒径为(197±32)nm,载药量(48.79±4.47)ug/mg,包封率(94.34±3.32)%。结论上述优化条件稳定,试验的可重复性高。
OBJECTIVE: To prepare galactosylated albumin magnetic nanoparticles with stable magnetic properties and capable of carrying chemotherapeutic drugs, and to detect the magnetic properties, drug content, entrapment efficiency, particle size and other physical properties of the nanoparticles. Methods Orthogonal experimental design method was used to design the experiment. The four factors including the curing temperature, curing time, stirring speed and saccharification ratio were investigated by the three indexes of particle size, drug loading and entrapment efficiency of nanometer drug Optimal combination of the best combination and repeat the experiment to verify the stability of experimental conditions and experimental repeatability. Firstly, galactosylated albumin is prepared, and doxorubicin, galactosylated albumin and magnetic powder are mixed according to a certain proportion, and the drug-carrying nanoparticles are prepared through the processes of ultrasonic emulsification, heat denaturation curing and ether washing in the refined cotton oil, Adriamycin was extracted from the nanoparticles by ethanol extraction, and the content of doxorubicin was determined by UV spectrophotometer. The particle size was analyzed by laser particle size analyzer. Results The nanoparticles prepared under the optimal conditions showed an average particle size of (197 ± 32) nm and drug loading (48.79 ± 4.47) μg / mg and encapsulation efficiency (94.34 ± 3.32)%, respectively. Conclusion The above optimization conditions are stable and the test reproducibility is high.