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目的 检测microRNA-21 (miR-21)在大肠癌患者中的表达情况,并探讨miR-21对大肠癌SW480细胞增殖和侵袭的影响.方法 收集大肠癌患者标本32例,同时收取癌组织及相应的癌旁组织.通过荧光定量PCR(qRT-PCR)方法检测各组织中miR-21的表达水平.以脂质体为载体将miR-21抑制剂转染至SW480细胞,分别用MTT方法与Transwell实验检测转染后SW480细胞的增殖侵袭能力变化,利用qRT-PCR和免疫印迹实验检测第10号染色体同源缺失的磷酸酶及张力蛋白同源的基因(PTEN) mRNA和蛋白水平的改变.结果 miR-21在32例患者癌组织及正常黏膜组织中的表达量分别为(1.57±0.79)与(0.56±0.32),差异有统计学意义(P＜0.01).下调miR-21的表达能够显著抑制SW480细胞的增殖和侵袭能力,PTEN mRNA和蛋白的表达水平均显著升高(P＜0.01).结论 microRNA-21在大肠癌中高表达,下调microRNA-21表达抑制大肠癌SW480细胞增殖和侵袭可能与上调PTEN表达相关.“,”Objective To examine the expression of microRNA-21(miR-21) in colorectal cancer,and to investigate the effects of miR-21 on the proliferation and invasion of SW480 cell line.Methods The expression of miR-21 was detected by quantitative real-time PCR(qRT-PCR) in 32 colorectal cancer tissues and their corresponding adjacent tissues.The miR-21 inhibitor was transfected into SW480 cells with Lipofectamine,and then proliferation and invasion ability of SW480 cells were detected by MTT method and transwell cell invasion assay,qRT-PCR and Western blot were used to examine the mRNA and protein expression of phosphatase and tensin homolog deleted on chromosome ten gene (PTEN) respectively.Results The expression level of miR-21 in the carcinoma tissues and normal tissues of 32 patients were (1.57 ± 0.79) and (0.56 ± 0.32) respectively,and with significant difference statistically(P＜0.01),the proliferation and invasion ability of SW480 cells were markedly inhibited by the inhibition of miR-21,and the mRNA and protein expression of PTEN was upregulated significantly too(P＜0.01).Conclusion miR-21 was overexpressed in colorectal cancer,the inhibition effect of miR-21 inhibitor on the proliferation and invasion ability of SW480 cells might be related to the upregulation of PTEN expression.