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目的:探讨两种产地秦岭柴胡不同提取物对D-氨基半乳糖致急性肝损伤小鼠的保护作用,并初步筛选最佳提取工艺和活性部位。方法:将小鼠分为9组:空白对照组,半乳糖胺模型组,联苯双酯滴丸(150 mg.kg-1),秦岭柴胡1号样品水提组、65%醇提组、95%醇提组,秦岭柴胡2号样品水提组、65%醇提组、95%醇提组。剂量为5.0 g.kg-1,灌胃给药,给药体积20 mL.kg-1,模型组和空白组分别灌胃给予同体积生理盐水,每天1次,连续给药7 d后,采取小鼠腹腔注射D-氨基半乳糖盐酸盐溶液造模,16 h后眼静脉采血,测定血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)活性;同时测定肝匀浆中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果:2种产地秦岭柴胡的水提组、65%醇提组、95%醇提组均能不同程度降低CCl4模型小鼠血清ALT,AST活性,降低肝脏MDA的含量,增强SOD活性,其中以东太白产秦岭柴胡65%醇提物作用最强(P<0.01,P<0.05)。结论:秦岭柴胡对D-氨基半乳糖盐酸盐所致小鼠急性肝损伤具有较明显的保护作用,其保肝作用与柴胡皂苷a,d含量存在一定的量效关系。
OBJECTIVE: To investigate the protective effect of different extracts of Bupleurum chinense of Qinling and Bupleurum on mice with acute hepatic injury induced by D-galactosamine and to screen the optimal extraction technology and active site. Methods: The mice were divided into 9 groups: blank control group, galactosamine model group, bifendate dropping pills (150 mg.kg-1), Qinhuai Bupleurum No.1 sample water extraction group, 65% alcohol extraction group , 95% ethanol extraction group, Qinling Bupleurum 2 sample water extraction group, 65% ethanol extraction group, 95% ethanol extraction group. The rats in the model group and the blank group were given the same volume of normal saline by gavage once a day for 7 days after continuous administration for 7 days Mice were intraperitoneally injected with D-galactosamine hydrochloride solution, and blood was collected from the ophthalmic vein 16 hours later. ALT and AST levels in serum were determined. Malondialdehyde (MDA) ) Content and superoxide dismutase (SOD) activity. Results: The water extract group, 65% ethanol extract group and 95% alcohol extract group of Bupleurum chinense L. from two producing areas all reduced the serum ALT and AST activities, reduced the content of MDA and increased the activity of SOD in CCl4 mice The effect of 65% ethanol extract of Qintailing Bupleurum on the east was the strongest (P <0.01, P <0.05). Conclusion: Qinling Bupleurum has a significant protective effect on acute hepatic injury induced by D-galactosamine hydrochloride in mice, and its hepatoprotective effect has some dose-effect relationship with that of saikosaponins a and d.